Shay A, Connolly T, Smith D N, Crean G L
Department of Immunology and Infectious Diseases, Pfizer Central Research, Groton, CT 06340.
Agents Actions. 1989 Jun;27(3-4):361-4. doi: 10.1007/BF01972823.
Two groups of naive guinea pigs were exposed to different LTB4 aerosol solutions: LTB4/saline and LTB4/BSA. Control animals were exposed to saline alone and saline/BSA. The LTB4/BSA aerosol was the most effective in recruiting leukocytes, with the maximal numbers of cells in the lung tissue at 6 hrs. Light microscopy/TEM revealed marked differences in eosinophil morphology in saline/BSA and LTB4/BSA aerosol treated animals. Occasional eosinophils in the submucosa of controls, had few pseudopodia, few cytoplasmic vesicles, and intact granules. In LTB4 treated animals, there were numerous eosinophils in the bronchial mucosa and submucosa with numerous pseudopodia, numerous cytoplasmic vesicles, and light crystalline cores.
两组未经处理的豚鼠暴露于不同的LTB4气雾剂溶液中：LTB4/生理盐水和LTB4/牛血清白蛋白。对照动物仅暴露于生理盐水和生理盐水/牛血清白蛋白。LTB4/牛血清白蛋白气雾剂在募集白细胞方面最有效，在6小时时肺组织中的细胞数量最多。光学显微镜/透射电子显微镜显示，在生理盐水/牛血清白蛋白和LTB4/牛血清白蛋白气雾剂处理的动物中，嗜酸性粒细胞形态存在明显差异。对照组黏膜下层偶尔出现的嗜酸性粒细胞，伪足少，胞质小泡少，颗粒完整。在LTB4处理的动物中，支气管黏膜和黏膜下层有大量嗜酸性粒细胞，有大量伪足、大量胞质小泡和浅色结晶核心。
