Nagy L, Lee T H, Goetzl E J, Pickett W C, Kay A B
Clin Exp Immunol. 1982 Mar;47(3):541-7.
The lipoxygenase products of arachidonic acid, 5-HPETE, 5-HETE, LTB4, LTC4 and LTD4, were examined for their capacity to enhance the expression of complement (C3b) receptors and to evoke chemotaxis of human neutrophils and eosinophils. With the exception of LTD4 all gave enhancement of C3b receptors. LTB4 and LTC4 enhanced over the concentration range 10(-7) to 10(-11) moles/l, and 5-HETE and 5-HPETE from 5 X 10(-6) to 5 X 10(-10) moles/l. The rank order of activity, as assessed by the magnitude of enhancement, was LTB4 (concentration for maximal effect = 10(-7) moles/l) greater than 5-HETE (5 X 10(-7)) greater than 5-HPETE (5 X 10(-6)) greater than LTC4 (10(-9)). High dose inhibition was observed with LTC4 and 5-HETE. Chemotaxis experiments performed in parallel over the same concentration ranges indicated that neither neutrophils nor eosinophils migrated towards LTC4 or LTD4. However, LTB4 evoked chemotaxis with a linear dose response from 10(-9) to 10(-7) moles/l and 5-HPETE and 5-HETE from 5 X 10(-8) to 5 X 10(-6) moles/l. At 10(-7) moles/l LTB4 was approximately 6 and 8 X more potent in chemotaxis than 5-HPETE and 5-HETE respectively. In general, complement receptor enhancement and chemotaxis of eosinophils were similar to that observed with neutrophils and did not vary with the patient source.
对花生四烯酸的脂氧合酶产物5 - HPETE、5 - HETE、LTB4、LTC4和LTD4进行了检测，以考察它们增强补体（C3b）受体表达以及引起人中性粒细胞和嗜酸性粒细胞趋化性的能力。除LTD4外，其他产物均能增强C3b受体表达。LTB4和LTC4在10^(-7)至10^(-11)摩尔/升的浓度范围内增强受体表达，5 - HETE和5 - HPETE在5×10^(-6)至5×10^(-10)摩尔/升的浓度范围内增强受体表达。根据增强程度评估的活性顺序为：LTB4（最大效应浓度 = 10^(-7)摩尔/升）＞5 - HETE（5×10^(-7)）＞5 - HPETE（5×10^(-6)）＞LTC4（10^(-9)）。观察到LTC4和5 - HETE存在高剂量抑制作用。在相同浓度范围内并行进行的趋化性实验表明，中性粒细胞和嗜酸性粒细胞均未向LTC4或LTD4迁移。然而，LTB4在10^(-9)至10^(-7)摩尔/升的浓度范围内引起趋化性，呈线性剂量反应，5 - HPETE和5 - HETE在5×10^(-8)至5×10^(-6)摩尔/升的浓度范围内引起趋化性。在10^(-7)摩尔/升时，LTB4在趋化性方面的效力分别比5 - HPETE和5 - HETE强约6倍和8倍。一般来说，嗜酸性粒细胞的补体受体增强和趋化性与中性粒细胞相似，且不随患者来源而变化。
