Psychoyos S, Uziel-Fusi S
Research Department, Pharmaceuticals Division Ciba-Geigy Corporation Summit, NJ 07901.
Agents Actions. 1989 Jun;27(3-4):380-4. doi: 10.1007/BF01972828.
Under comparable conditions (90 min incubation in 2% albumin buffer) using 3 micron pore cellulose nitrate filters and the multiwell cap procedure (Evans et al., Bioscience Reports 6:1041, 1986), C5a was more potent than LTB4 as a chemoattractant (EC'50 s = 0.5 and 4 nM) and caused 5 times as many cells to completely traverse the filter. During the 90 min incubation, no cells traversed the filter in the absence of chemoattractant. The cap assay was modified to study migration of cells within the filter. During the 30 min incubation, EC50 values for C5a and LTB4 were comparable (1.2 and 1.6 nM) in causing cells to enter the filters but C5a was superior in causing the cells to move as measured by the mean distance of migration (EC'50 s = 0.25 and 1.8 nM). Our studies support the view that LTB4 acts primarily to cause cell adhesion (and penetration) of the endothelium and that C5a plays a major role in cell migration (McMillan and Foster, Agents and Actions 24: 114, 1988).
在可比条件下（于2%白蛋白缓冲液中孵育90分钟），使用3微米孔径的硝酸纤维素滤膜及多孔盖方法（Evans等人，《生物科学报告》6:1041，1986年），作为趋化因子，C5a比LTB4更具效力（半数有效浓度分别为0.5和4 nM），且能使完全穿过滤膜的细胞数量多出5倍。在90分钟的孵育过程中，若无趋化因子，无细胞穿过滤膜。对盖试验进行了改进以研究滤膜内细胞的迁移。在30分钟的孵育过程中，C5a和LTB4促使细胞进入滤膜的半数有效浓度值相当（分别为1.2和1.6 nM），但就细胞迁移的平均距离而言，C5a促使细胞移动的能力更强（半数有效浓度分别为0.25和1.8 nM）。我们的研究支持以下观点：LTB4主要作用是引起内皮细胞的黏附（及穿透），而C5a在细胞迁移中起主要作用（McMillan和Foster，《药物与作用》24: 114，1988年）。
