Cornall Alyssa M, Phillips Samuel, Cummins Eleanor, Garland Suzanne M, Tabrizi Sepehr N
Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia.
Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia.
J Virol Methods. 2015 Mar;214:10-4. doi: 10.1016/j.jviromet.2014.12.007. Epub 2014 Dec 18.
In populations where the prevalence of vaccine-targeted HPV types has been reduced significantly due to widespread vaccination of the target population, the sensitivity of some consensus PCR-based assays to detect remaining HPV types may be altered, leading to misrepresentations of prevalence. Importantly, this may lead to false indications of type replacement in vaccinated populations. To assess whether excess vaccine-targeted HPV DNA resulted in reduced detection of other genotypes on the Roche HPV linear array genotype assay, simulated samples containing 1000 copies of one or two high-risk HPV DNA genomes in the presence and the absence of 10,000 copies of the HPV16 genome were tested. HPV16 alone did not affect detection of other high-risk genotypes; however when HPV16 and an additional genotype were present, detection of HPV31, 33, 51 or 59 was impeded, indicating potential for misrepresentation of population-based prevalence of these genotypes and false evidence for type replacement following vaccination.
在目标人群广泛接种疫苗后，疫苗靶向的人乳头瘤病毒（HPV）型别流行率显著降低的人群中，一些基于共识PCR的检测方法检测剩余HPV型别的敏感性可能会改变，从而导致流行率的误报。重要的是，这可能导致在接种疫苗人群中出现型别替代的错误指示。为了评估过量的疫苗靶向HPV DNA是否会导致罗氏HPV线性阵列基因分型检测中其他基因型的检测减少，对含有1000个拷贝的一种或两种高危HPV DNA基因组的模拟样本进行了检测，样本中分别存在和不存在10000个拷贝的HPV16基因组。单独的HPV16并不影响其他高危基因型的检测；然而，当同时存在HPV16和另一种基因型时，HPV31、33、51或59的检测受到阻碍，这表明这些基因型基于人群的流行率可能被误报，并且在接种疫苗后可能出现型别替代的错误证据。
