微小RNA-134通过靶向POGLUT1和Notch信号通路蛋白抑制子宫内膜癌干细胞。
MicroRNA-134 suppresses endometrial cancer stem cells by targeting POGLUT1 and Notch pathway proteins.
作者信息
Gao Yongtao, Liu Te, Huang Yongyi
机构信息
International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai 200030, China.
International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai 200030, China; Shanghai Tenth People's Hospital, Medical School, Tongji University, Shanghai 200072, China; Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, China.
出版信息
FEBS Lett. 2015 Jan 16;589(2):207-14. doi: 10.1016/j.febslet.2014.12.002. Epub 2014 Dec 17.
We aimed to ascertain the role of microRNAs (miRNAs) in regulating human endometrial cancer stem cells (HuECSCs). The expression level of miRNA-134 (miR-134), a member of the DLK1-DIO3 genomic imprinted miRNA cluster, differed significantly between HuECSCs and human endometrial cancer cells (HuECCs). miR-134 inhibited HuECSCs proliferation and migration by targeting protein O-glucosyltransferase 1 (POGLUT1) expression. Exogenous miR-134 overexpression downregulated POGLUT1 and Notch pathway proteins in HuECSCs in vitro. miR-134 overexpression affected the G2/M phase of HuECSCs and suppressed the growth of xenograft tumours formed. Thus, endogenous miR-134 regulation in HuECSCs may suppress tumourigenesis in human endometrial carcinoma.
我们旨在确定微小RNA（miRNA）在调节人子宫内膜癌干细胞（HuECSCs）中的作用。DLK1-DIO3基因组印记miRNA簇成员之一的miRNA-134（miR-134）在HuECSCs和人子宫内膜癌细胞（HuECCs）之间的表达水平存在显著差异。miR-134通过靶向蛋白O-葡萄糖基转移酶1（POGLUT1）的表达来抑制HuECSCs的增殖和迁移。外源性miR-134过表达在体外下调了HuECSCs中POGLUT1和Notch信号通路蛋白的表达。miR-134过表达影响了HuECSCs的G2/M期，并抑制了异种移植肿瘤的生长。因此，HuECSCs中内源性miR-134的调节可能会抑制人子宫内膜癌的肿瘤发生。
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