Clayton F, Klein E B, Kotler D P
Department of Pathology, St Luke's Roosevelt Hospital Center, New York, NY.
Arch Pathol Lab Med. 1989 Oct;113(10):1124-6.
Endoscopic colonic biopsy specimens from 34 patients with acquired immunodeficiency syndrome and six patients without acquired immunodeficiency syndrome (3 were human immunodeficiency virus-seropositive and 3 were human immunodeficiency virus-seronegative) were examined by in situ hybridization for evidence of cytomegalovirus colitis and the results were compared with histologic examinations and viral cultures. In situ hybridization was positive in 22 of 25 patients with acquired immunodeficiency syndrome with histologic evidence of cytomegalovirus colitis. By our interpretation, 15 patients without cytomegalovirus colitis histologically all had negative hybridization studies. No correlation was found between in situ hybridization and viral culture results. In situ hybridization is a useful confirmatory test when the histologic changes are suspicious for cytomegalovirus but not considered diagnostic; it will only rarely demonstrate staining in a case considered negative histologically.
对34例获得性免疫缺陷综合征患者和6例非获得性免疫缺陷综合征患者（3例人类免疫缺陷病毒血清学阳性，3例人类免疫缺陷病毒血清学阴性）的内镜结肠活检标本进行原位杂交，以检测巨细胞病毒性结肠炎的证据，并将结果与组织学检查和病毒培养结果进行比较。在25例有巨细胞病毒性结肠炎组织学证据的获得性免疫缺陷综合征患者中，22例原位杂交呈阳性。根据我们的判断，15例组织学上无巨细胞病毒性结肠炎的患者，杂交研究均为阴性。原位杂交与病毒培养结果之间未发现相关性。当组织学改变怀疑为巨细胞病毒感染但不能确诊时，原位杂交是一种有用的确诊试验；在组织学诊断为阴性的病例中，原位杂交很少显示染色阳性。
