López-Muñoz Gerardo A, Antonio-Pérez Aurora, Díaz-Reyes J
Química Aromática S.A., Río Grande S/N, col. Santa Catarina, Acolman, C.P. 55875 Mexico State, Mexico.
Instituto Tecnológico y de Estúdios Superiores de Monterrey-Campus Estado de México, Carretera Lago de Guadalupe Km. 3.5, col. Margarita Maza de Juarez, Atizapán de Zaragoza, C.P. 52926 Mexico State, Mexico.
Food Chem. 2015 May 1;174:104-9. doi: 10.1016/j.foodchem.2014.11.039. Epub 2014 Nov 14.
A general theory of thermal wave resonant cavity photopyroelectric spectroscopy (TWRC-PPE) was recently proposed by Balderas-López (2012) for the thermo-optical characterisation of substances in a condensed phase. This theory is used to quantify the total carotenoids and chlorophylls in several folded and un-folded citrus essential oils to demonstrate the viability of using this technique as an alternative analytical method for the quantification of total pigments in citrus oils. An analysis of variance (ANOVA) reveals significant differences (p < 0.05) among the means of optical absorption coefficient data for the folding degree and fruit type in citrus oils. The experimental results show that TWRC-PPE spectroscopy can be used to quantify concentrations up to five times higher of total carotenoids and chlorophylls in citrus oils than UV-Vis spectroscopy without sample preparation or dilution. The optical limits of this technique and possible interference are also described.
巴尔德拉斯 - 洛佩斯（2012年）最近提出了一种热波共振腔光热释电光谱学（TWRC - PPE）的通用理论，用于凝聚相中物质的热光学表征。该理论用于量化几种折叠和未折叠的柑橘精油中的总类胡萝卜素和叶绿素，以证明使用该技术作为柑橘油中总色素定量分析替代方法的可行性。方差分析（ANOVA）表明，柑橘油中折叠程度和果实类型的光吸收系数数据均值之间存在显著差异（p < 0.05）。实验结果表明，TWRC - PPE光谱学可用于量化柑橘油中总类胡萝卜素和叶绿素的浓度，比紫外 - 可见光谱法高五倍，且无需样品制备或稀释。还描述了该技术的光学极限和可能的干扰。
