Nevorotin A I, Kull' M M
Arkh Patol. 1989;51(7):63-70.
Linear or dot-shaped lesions were inflicted on rat liver with Nd:YAG laser, and fine structural alterations of hepatocytes were studied in the specimens processed for an endoplasmic reticulum (ER) marker glucose-6-phosphatase (GP). 5-7 s after irradiation a severe cell damage and GP inhibition occurred near the lesions, with less injured cells located laterally. 24 hr later the zone of the necrosis increased markedly. An autolytic decomposition in the newly formed necrotic area was much more pronounced as compared to the area of the initial necrosis. Phagocytic resorption of the intensively irradiated tissue was retarded this explaining some clinical observations on the long-term healing after Nd:YAG laser surgery. Based on our observations the so-called contact regimen of the irradiation is recommended due to the small size of the initial necrosis produced with this method. The various patterns of cell injuries including some changes in ER and enzyme GP as its marker are described in detail.
用钕钇铝石榴石激光在大鼠肝脏上造成线性或点状损伤，并在经内质网（ER）标记物葡萄糖-6-磷酸酶（GP）处理的标本中研究肝细胞的细微结构变化。照射后5-7秒，损伤部位附近出现严重的细胞损伤和GP抑制，侧面的细胞损伤较轻。24小时后，坏死区域明显扩大。与初始坏死区域相比，新形成的坏死区域的自溶分解更为明显。高强度照射组织的吞噬吸收受到抑制，这解释了钕钇铝石榴石激光手术后长期愈合的一些临床观察结果。基于我们的观察结果，由于这种方法产生的初始坏死面积较小，建议采用所谓的接触照射方式。详细描述了包括内质网的一些变化以及作为其标记物的酶GP在内的各种细胞损伤模式。
