Machiulaĭtis R R, Zel'tser G L, Zhloba A A, Pluzhnikov M S, Nevorotin A I
Biull Eksp Biol Med. 1991 Feb;111(2):217-9.
Small tissue strips were excised from rat liver and exposed to extensive irradiation with Nd:YAG laser followed by homogenization and spectrophotometric detection of 5% TCA-soluble protein fractions (oligopeptides) in both supernatants and 5NKOH-soluble sediments of the irradiated specimens. Trypsin along, or with chemotrypsin was added to the specimens to evaluate their proteolytic resistance during and after incubation for 24 h in the proteinase(s). The results showed that Nd:YAG laser irradiation resulted in significantly more oligopeptides following either schedule of the proteolysis employed in both supernatants and sediments as compared to the unaffected proteolytically tested specimens. At the same time a proteolytically resistant 5% TCA-insoluble fraction was elicited which belonged to proteins and, judging by the parallel ultrastructural data, was located in the cellular membranes. The probable mechanisms of the observed protein modifications, along with some pertinent clinical implications of Nd:YAG laser surgery, are considered.
从小鼠肝脏切下小块组织条，用Nd:YAG激光进行广泛照射，然后进行匀浆，并对照射标本的上清液和5N KOH可溶性沉淀物中的5%三氯乙酸可溶性蛋白质组分（寡肽）进行分光光度检测。将胰蛋白酶单独或与胰凝乳蛋白酶一起加入标本中，以评估其在蛋白酶中孵育24小时期间及之后的抗蛋白水解能力。结果表明，与未受影响的蛋白水解测试标本相比，Nd:YAG激光照射后，在两种蛋白酶解方案下，上清液和沉淀物中产生的寡肽明显更多。同时，诱导出了一种抗蛋白水解的5%三氯乙酸不溶性组分，其属于蛋白质，根据平行的超微结构数据判断，位于细胞膜中。文中考虑了观察到的蛋白质修饰的可能机制，以及Nd:YAG激光手术的一些相关临床意义。
