Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China; College of Pharmacy, South Central University for Nationalities, Wuhan 430074, China.
Food Chem. 2015 May 1;174:233-9. doi: 10.1016/j.foodchem.2014.11.046. Epub 2014 Nov 14.
A novel method was developed based on capillary electrophoresis with indirect laser-induced fluorescence detection for the determination of alditols including xylitol, sorbitol, arabinitol and mannitol. The baseline separation was achieved with a capillary with 30 μm inner diameter and running buffer (pH 9.6) composed of 40 mM sodium tetraborate, 5% (v/v) methanol, 10(-5)M sodium fluorescein as background fluorescence. Calibration curves (0.2-2.0 mg mL(-1)) showed good linear correlations (r>0.999). The detection limits of the alditols were in the range of 19.0-24.4 μg mL(-1). The intra-day and inter-day RSDs for migration times and peak areas were less than 1.6% and 4.3%, respectively. The average recoveries were in the range of 99.6-105.5%. The developed method was successfully applied to determination of alditols in different real samples, and the results were compared with those of capillary electrophoresis with indirect ultraviolet detection method.
一种新的方法是基于毛细管电泳与间接激光诱导荧光检测用于测定糖醇包括木糖醇、山梨醇、阿拉伯糖醇和甘露醇。基线分离是用毛细管内径 30μm 和运行缓冲液（pH9.6）组成的 40 毫米硼酸钠，5%（体积/体积）甲醇，10（-5）M 荧光素钠作为背景荧光。校准曲线（0.2-2.0 毫克毫升（-1））显示了良好的线性关系（r 大于 0.999）。糖醇的检测限在 19.0-24.4μg毫升（-1）的范围内。日内和日间迁移时间和峰面积的 RSD 小于 1.6%和 4.3%，分别。平均回收率在 99.6-105.5%的范围内。该方法成功地应用于不同实际样品中糖醇的测定，并与间接紫外检测法的毛细管电泳结果进行了比较。
