伴刀豆球蛋白A刺激的大鼠淋巴细胞中AMP/腺苷底物循环的速率。
The rate of the AMP/adenosine substrate cycle in concanavalin-A-stimulated rat lymphocytes.
作者信息
Szondy Z, Newsholme E A
机构信息
Department of Biochemistry, University of Oxford, U.K.
出版信息
Biochem J. 1989 Aug 1;261(3):739-42. doi: 10.1042/bj2610739.
The effect of adenosine on the metabolism of prelabelled adenine nucleotides was investigated in concanavalin-A-stimulated rat lymphocytes. Adenosine in the presence of the adenosine deaminase inhibitor, deoxycoformycin, caused a 2-fold increase in the ATP concentration. This effect was, in part, countereacted by an increased rate of adenine nucleotide catabolism, which could be explained by a stimulation of AMP deaminase (EC 3.5.4.6). At the same time a continuous rate of labelled adenosine production was found, which was not affected by the increased ATP concentration and which could only be detected by the trapping effect of a high concentration of added unlabelled adenosine. It is concluded that the rate of the substrate cycle between AMP and adenosine is low (1.9 +/- 0.2 nmol/h per 10(7) cells) in comparison to the rate of AMP deamination.
在伴刀豆球蛋白A刺激的大鼠淋巴细胞中研究了腺苷对预先标记的腺嘌呤核苷酸代谢的影响。在腺苷脱氨酶抑制剂脱氧助间型霉素存在的情况下，腺苷使ATP浓度增加了2倍。这种作用部分被腺嘌呤核苷酸分解代谢速率的增加所抵消，这可以通过AMP脱氨酶（EC 3.5.4.6）的刺激来解释。同时发现了标记腺苷的持续产生速率，其不受ATP浓度增加的影响，并且只能通过添加高浓度未标记腺苷的捕获作用来检测。得出的结论是，与AMP脱氨速率相比，AMP和腺苷之间底物循环的速率较低（每10^7个细胞1.9±0.2 nmol/h）。
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