Falatach Rebecca, McGlone Cameron, Al-Abdul-Wahid M Sameer, Averick Saadyah, Page Richard C, Berberich Jason A, Konkolewicz Dominik
Department of Chemical, Paper and Biomedical Engineering, Miami University, 650 E High St, Oxford, OH 45056, USA.
Chem Commun (Camb). 2015 Mar 28;51(25):5343-6. doi: 10.1039/c4cc09287b.
Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein-oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.
通过采用可逆加成-断裂链转移（RAFT）聚合的“接枝到”和“接枝自”方法相结合，将亲水性聚合物连接到溶菌酶上。合成了一种亲水性低聚物，并将其连接到蛋白质上。蛋白质-低聚物杂化物含有RAFT端基，能够在溶液中进行链延伸。溶菌酶在整个过程中保持活性。
