建立一种表现出成骨细胞表型特征的具有骨诱导性的小鼠骨肉瘤克隆细胞系。

Establishment of an osteoinductive murine osteosarcoma clonal cell line showing osteoblastic phenotypic traits.

作者信息

Tsuda T, Masuhara K, Yoshikawa H, Shimizu N, Takaoka K

机构信息

Department of Orthopedic Surgery, Osaka University Medical School, Japan.

出版信息

Bone. 1989;10(3):195-200. doi: 10.1016/8756-3282(89)90053-7.

DOI:10.1016/8756-3282(89)90053-7

PMID:2553082

Abstract

A clonal cell line, BFO-6, was established in culture from a Dunn osteosarcoma cell line (BFO) and characterized on the basis of bone-inducing activity, alkaline phosphatase activity, PTH sensitive cAMP production and tumorigenicity. Lyophilized pellets of devitalized cells, when implanted into the back muscles of allogenic host mice, consistently elicited heterotopic bone formation at 3 weeks postimplantation. BFO-6 cells were found to be rich in alkaline phosphatase activity and showed a significant response to h-PTH stimulation. The doubling time in the logarithmic phase was 17.2 h and the cells revealed an acrocentric karyotype. Subcutaneous transplantation of cells (1 x 10(7) cells) into a C3H mouse resulted in the production of a tumor with histological features of osteosarcoma. This tumor also retained bone-inducing activity and high alkaline phosphatase activity.

摘要

从邓恩骨肉瘤细胞系（BFO）中培养建立了一种克隆细胞系BFO-6，并根据骨诱导活性、碱性磷酸酶活性、甲状旁腺激素敏感的环磷酸腺苷生成以及致瘤性对其进行了表征。将失活细胞的冻干微球植入同种异体宿主小鼠的背部肌肉后，在植入后3周时始终引发异位骨形成。发现BFO-6细胞富含碱性磷酸酶活性，并对人甲状旁腺激素刺激有显著反应。对数期的倍增时间为17.2小时，细胞显示出近端着丝粒核型。将细胞（1×10⁷个细胞）皮下移植到C3H小鼠体内会产生具有骨肉瘤组织学特征的肿瘤。该肿瘤也保留了骨诱导活性和高碱性磷酸酶活性。