源自威尔姆斯瘤的培养细胞中11号染色体短臂等位基因的缺失。

Brown K W, Shaw A P, Poirier V, Tyler S J, Berry P J, Mott M G, Maitland N J

Department of Pathology, Medical School, University Walk, Bristol, UK.

Br J Cancer. 1989 Jul;60(1):25-9. doi: 10.1038/bjc.1989.213.

Cell cultures have been produced from five Wilms' tumours. All cultures had a finite lifespan and a pattern of antigen expression which indicated that the cells were derived from the differentiated components of the tumours. No cells showed any of the expected characteristics of the putative Wilms' tumour stem cell. Nevertheless, in both cases where the original tumours showed a loss of heterozygosity at chromosome 11p alleles, the cultured cells also demonstrated a loss of heterozygosity. Thus these cell cultures definitely originated from Wilms' tumour tissue. The results demonstrate that cell cultures can be produced from the differentiated tissues present in Wilms' tumours and that these non-immortal cells show no 'transformed' phenotype, even though they possess the genetic changes present in the original tumour.

已从五个肾母细胞瘤中培养出细胞。所有培养物都有有限的寿命和抗原表达模式，这表明这些细胞源自肿瘤的分化成分。没有细胞表现出假定的肾母细胞瘤干细胞的任何预期特征。然而，在原始肿瘤在11号染色体p等位基因处显示杂合性缺失的两个病例中，培养的细胞也表现出杂合性缺失。因此，这些细胞培养物肯定源自肾母细胞瘤组织。结果表明，可以从肾母细胞瘤中存在的分化组织中培养出细胞，并且这些非永生细胞即使具有原始肿瘤中存在的基因变化，也没有表现出“转化”表型。