Osborn M, Debus E, Weber K
Eur J Cell Biol. 1984 May;34(1):137-43.
Previously described vimentin monoclonal antibodies recognize other cellular or intermediate filament proteins in addition to vimentin. In contrast the set of murine monoclonal antibodies described here and elicited using porcine vimentin as antigen appear specific for vimentin. All seven antibodies react only with vimentin in "immunoblot" analysis on gel electrophoretically separated polypeptides from total cell extracts. They do not recognize other closely related intermediate filament proteins including desmin and GFA (glial fibrillary acidic protein). Immunofluorescence microscopy on tissue sections shows a positive reaction in connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. Typical intermediate filament profiles are documented in fibroblasts and in other cultured cells known to contain vimentin. Although all antibodies react with human and porcine vimentin some show species-specific restriction when rat, mouse and chicken vimentin are used. These results, together with different IgG types and certain aspects of the staining patterns, allow a subdivision of the antibodies. The vimentin antibodies described here complete a set of monoclonal antibodies each specific for only one intermediate filament protein type. Their reaction on human material allows their use in pathology to determine the histogenetic origin of neoplasms.
先前描述的波形蛋白单克隆抗体除了能识别波形蛋白外，还能识别其他细胞或中间丝蛋白。相比之下，本文描述的以猪波形蛋白作为抗原产生的一组鼠单克隆抗体似乎对波形蛋白具有特异性。在对来自全细胞提取物的经凝胶电泳分离的多肽进行的“免疫印迹”分析中，所有七种抗体仅与波形蛋白发生反应。它们不识别其他密切相关的中间丝蛋白，包括结蛋白和胶质纤维酸性蛋白（GFA）。组织切片的免疫荧光显微镜检查显示，在结缔组织、内皮细胞、血管平滑肌细胞和星形胶质细胞中呈阳性反应。在成纤维细胞和其他已知含有波形蛋白的培养细胞中记录到了典型的中间丝形态。尽管所有抗体都能与人及猪波形蛋白发生反应，但当使用大鼠、小鼠和鸡的波形蛋白时，有些抗体表现出种属特异性限制。这些结果，连同不同的IgG类型和染色模式的某些方面，使得这些抗体可以细分。本文描述的波形蛋白抗体完善了一组单克隆抗体，每种抗体仅对一种中间丝蛋白类型具有特异性。它们在人体材料上的反应使其可用于病理学中确定肿瘤的组织发生起源。
