Hechler V, Gobaille S, Maitre M
Centre de Neurochimie du CNRS, Strasbourg, France.
Brain Res Bull. 1989 Jul-Aug;23(1-2):129-35. doi: 10.1016/0361-9230(89)90171-8.
Quantitative autoradiography using [3H] gamma-hydroxybutyrate was used in combination with anatomic and neurotoxic lesions to localize the gamma-hydroxybutyrate (GHB) receptors in the striatum and hippocampus of rat brain. 6-Hydroxydopamine (6-OHDA) lesions of the nigro-striatal pathway failed to reduce [3H] gamma-hydroxybutyrate binding in the striatum. In contrast, kainic acid (KA) lesions of the caudate-putamen (CPu) resulted in about 45% loss of binding. For hippocampus, lesions of septo-hippocampal pathway did not modify receptor density but intrahippocampal kainic acid injection largely attenuated (50%) [3H] GHB binding. These results demonstrate that gamma-hydroxybutyrate receptors in the CPu and dorsal hippocampus are principally located on intrinsic neurons which may participate in the functional expression of the role gamma-hydroxybutyrate has in these structures.
利用[3H]γ-羟基丁酸进行定量放射自显影，并结合解剖学和神经毒性损伤，来定位大鼠脑纹状体和海马体中的γ-羟基丁酸（GHB）受体。黑质-纹状体通路的6-羟基多巴胺（6-OHDA）损伤未能降低纹状体中[3H]γ-羟基丁酸的结合。相反，尾状核-壳核（CPu）的 kainic 酸（KA）损伤导致约45%的结合丧失。对于海马体，隔海马通路的损伤并未改变受体密度，但海马体内注射 kainic 酸可大幅减弱（50%）[3H]GHB 的结合。这些结果表明，CPu 和背侧海马体中的γ-羟基丁酸受体主要位于内在神经元上，这些神经元可能参与γ-羟基丁酸在这些结构中所起作用的功能表达。
