Vayer P, Maitre M
Centre de Neurochimie du C.N.R.S. and INSERM U44, Strasbourg, France.
Neurosci Lett. 1988 Apr 22;87(1-2):99-103. doi: 10.1016/0304-3940(88)90152-8.
gamma-Hydroxybutyrate (GHB), an endogenous compound in mammalian brain which possesses important neuroregulatory properties, has been proposed as a neurotransmitter. The present report concerns the heterogeneous regional characteristics of depolarization-induced gamma-[3H]hydroxybutyrate ([3H]GHB) release in rat brain slices. The Ca2+-dependent component of [3H]GHB release in cerebellum and pons-medulla, areas with low concentrations of GHB binding sites, is only about 23% of that in hippocampus, striatum and frontoparietal cortex, all areas rich in binding sites. In addition, veratridine-induced release of [3H]GHB in cerebellum and pons-medulla is only 16% of that measured in hippocampus, striatum and frontoparietal cortex. These findings add support to the suggested neurotransmitter role of GHB in the forebrain and suggest a purely metabolic role for this compound in more posterior regions of brain where both binding sites and Ca2+-dependent release mechanisms are absent.
γ-羟基丁酸（GHB）是哺乳动物大脑中的一种内源性化合物，具有重要的神经调节特性，已被提出作为一种神经递质。本报告关注大鼠脑片中去极化诱导的γ-[³H]羟基丁酸（[³H]GHB）释放的异质性区域特征。在小脑和脑桥-延髓中，GHB结合位点浓度较低的区域，[³H]GHB释放的Ca²⁺依赖性成分仅约为海马体、纹状体和额顶叶皮质（所有富含结合位点的区域）的23%。此外，藜芦碱诱导的小脑和脑桥-延髓中[³H]GHB的释放仅为海马体、纹状体和额顶叶皮质中测量值的16%。这些发现为GHB在前脑所提示的神经递质作用提供了支持，并表明该化合物在大脑更靠后的区域（缺乏结合位点和Ca²⁺依赖性释放机制）中仅具有纯粹的代谢作用。
