Qsaib Said, Mateus Nuno, Ikbal Fatima Ez-zohra, Rifai Lala Aicha, De Freitas Victor, Koussa Tayeb
Nat Prod Commun. 2014 Nov;9(11):1569-72.
The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and ε-viniferin had similar levels in buds and internodes while six other compounds identified had higher levels in buds.
采用反相高效液相色谱结合二极管阵列检测（HPLC-DAD）和质谱（LC-MS）的方法，对酿酒葡萄品种梅洛休眠芽和节间的粗甲醇提取物中的酚类化合物进行测定。该方法无需纯化或分级分离即可鉴定出9种酚类化合物。这9种化合物分为三组：原花青素、黄酮醇和芪类化合物。通过在280nm至320nm不同波长下的HPLC-DAD检测，可以估算这些化合物的浓度。该方法首次实现了葡萄芽中多酚类化合物的表征和定量。与节间的结果比较表明，槲皮素、白藜芦醇四聚体和ε-葡萄素在芽和节间中的含量相似，而鉴定出的其他六种化合物在芽中的含量更高。
