Wyroba E
Department of Cell Biology, Nencki Institute of Experimental Biology, Warsaw, Poland.
Cell Biol Int Rep. 1989 Aug;13(8):667-78. doi: 10.1016/0309-1651(89)90043-x.
Bete-adrenergic agonists isoproterenol and norepinephrine enhanced phagocytosis in Paramecium. Stimulation was stereospecific, dose-dependent and inhibited by the beta-agonists propranolol and alprenolol. Phorbol ester and forskolin potentiated the stimulatory effect of catecholamines on Paramecium phagocytosis. The dansyl analogue of propranolol (DAPN) was used for fluorescent visualization of the beta-adrenergic receptor sites in Paramecium which have been found to be localized at the cell membrane and within the membrane of the nascent digestive vacuoles. The appearance of the characteristic fluorescent pattern has been blocked by 1-propranolol.
β-肾上腺素能激动剂异丙肾上腺素和去甲肾上腺素增强了草履虫的吞噬作用。刺激具有立体特异性、剂量依赖性，并被β-激动剂普萘洛尔和阿普洛尔抑制。佛波酯和福斯高林增强了儿茶酚胺对草履虫吞噬作用的刺激效果。普萘洛尔的丹磺酰类似物（DAPN）用于对草履虫中β-肾上腺素能受体位点进行荧光可视化，已发现这些位点位于细胞膜以及新生消化液泡的膜内。特征性荧光模式的出现已被1-普萘洛尔阻断。
