Boccara Charlotte N, Kjonigsen Lisa J, Hammer Ingvild M, Bjaalie Jan G, Leergaard Trygve B, Witter Menno P
Centre for Neural Computation, Kavli Institute for System Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Institute of Science and Technology IST, Klosterneuburg, Austria.
Hippocampus. 2015 Jul;25(7):838-57. doi: 10.1002/hipo.22407. Epub 2015 Jan 20.
The hippocampal region, comprising the hippocampal formation and the parahippocampal region, has been one of the most intensively studied parts of the brain for decades. Better understanding of its functional diversity and complexity has led to an increased demand for specificity in experimental procedures and manipulations. In view of the complex 3D structure of the hippocampal region, precisely positioned experimental approaches require a fine-grained architectural description that is available and readable to experimentalists lacking detailed anatomical experience. In this paper, we provide the first cyto- and chemoarchitectural description of the hippocampal formation and parahippocampal region in the rat at high resolution and in the three standard sectional planes: coronal, horizontal and sagittal. The atlas uses a series of adjacent sections stained for neurons and for a number of chemical marker substances, particularly parvalbumin and calbindin. All the borders defined in one plane have been cross-checked against their counterparts in the other two planes. The entire dataset will be made available as a web-based interactive application through the Rodent Brain WorkBench (http://www.rbwb.org) which, together with this paper, provides a unique atlas resource.
海马区由海马结构和海马旁区域组成，几十年来一直是大脑中研究最为深入的部分之一。对其功能多样性和复杂性的更深入理解，导致对实验程序和操作特异性的需求增加。鉴于海马区复杂的三维结构，精确的实验方法需要精细的结构描述，以便缺乏详细解剖学经验的实验人员能够获取和理解。在本文中，我们首次以高分辨率在冠状、水平和矢状三个标准切面，对大鼠海马结构和海马旁区域进行了细胞和化学结构描述。该图谱使用了一系列相邻切片，分别对神经元以及多种化学标记物质（特别是小白蛋白和钙结合蛋白）进行染色。在一个平面上定义的所有边界都已与另外两个平面上的对应边界进行了交叉核对。整个数据集将通过啮齿动物脑工作台（http://www.rbwb.org）作为基于网络的交互式应用程序提供，该工作台与本文一起提供了一个独特的图谱资源。
