Pratt S L, Calcatera S M, Stowe H M, Dimmick M A, Schrick F N, Duckett S K, Andrae J G
Department of Animal and Veterinary Sciences, Clemson University, Clemson, South Carolina, USA.
Department of Animal and Veterinary Sciences, Clemson University, Clemson, South Carolina, USA.
Theriogenology. 2015 Mar 1;83(4):662-9. doi: 10.1016/j.theriogenology.2014.10.031. Epub 2014 Nov 20.
The objectives of this study were to determine (1) the presence and expression levels of bovine prolactin receptor (PRLR) and prolactin-inducible protein (PIP) in bovine testis and epididymis, and (2) the presence and concentrations of prolactin (PRL) present in seminiferous fluid in bulls consuming diets with (E+) or without (E-) ergot alkaloids. Bulls (n = 8) were sacrificed after 126 days (group A) of E+ or E- treatment or 60 days after all bulls (n = 6) were switched to the E- ration (group B). End point and real-time quantitative reverse transcription-polymerase chain reaction and immunohistochemistry were conducted on testis and epididymis samples to establish the presence and relative expression of PRLR and PIP. Seminal fluid samples obtained from bulls consuming E- and E+ diets were subjected to RIA for PRL. Both PIP and PRLR were present in testis and epididymis as determined by reverse transcription-polymerase chain reaction and immunohistochemistry. Prolactin-inducible protein mRNA abundance was affected by time of slaughter in testis and epididymis head, respectively (P < 0.05). Prolactin receptor mRNA expression was affected by time of slaughter in the epididymis (P < 0.05) and differed in testis samples because of treatment (P < 0.05). Radioimmunoassay establishes the presence of PRL in seminal fluid; however, differences in the concentration of PRL over two separate studies were inconsistent, possibly because of differences in diet. The presence and localization of the PRLR are consistent with expression data reported for other species, and the presence of PIP and PRL in seminal fluid is consistent with data generated in humans.
（1）牛催乳素受体（PRLR）和催乳素诱导蛋白（PIP）在牛睾丸和附睾中的存在情况及表达水平；（2）采食含（E+）或不含（E-）麦角生物碱日粮的公牛精液中催乳素（PRL）的存在情况及浓度。在进行E+或E-处理126天后（A组，n = 8头公牛）或所有公牛（n = 6头）转为E-日粮60天后（B组），宰杀公牛。对睾丸和附睾样本进行终点和实时定量逆转录-聚合酶链反应以及免疫组织化学，以确定PRLR和PIP的存在及相对表达。从采食E-和E+日粮的公牛采集精液样本，进行PRL的放射免疫分析。通过逆转录-聚合酶链反应和免疫组织化学确定，PIP和PRLR均存在于睾丸和附睾中。催乳素诱导蛋白mRNA丰度在睾丸和附睾头部分别受屠宰时间的影响（P < 0.05）。催乳素受体mRNA表达在附睾中受屠宰时间的影响（P < 0.05），在睾丸样本中因处理方式不同而存在差异（P < 0.05）。放射免疫分析确定精液中存在PRL；然而，在两项独立研究中PRL浓度的差异并不一致，可能是由于日粮不同。PRLR的存在和定位与其他物种报道的表达数据一致，精液中PIP和PRL的存在与人类研究数据一致。
