Li Mingqian, He Xinyi, Liu Han, Fu Zhangwuke, He Xiangkang, Lu Xingmeng
Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Acta Biochim Biophys Sin (Shanghai). 2015 Feb;47(2):80-90. doi: 10.1093/abbs/gmu119. Epub 2014 Dec 22.
The flacherie disease in the silkworm is caused by the infectious flacherie virus (IFV). IFV relies on its 5' region of genomic RNA to recruit host-related factors to implement viral translation and replication. To identify host proteins bound to the 5'-region of IFV RNA and identify proteins important for its function, mass spectrometry was used to identify proteins from silkworm midgut extracts that were obtained using RNA aptamer-labeled 5' region of IFV RNA. We found 325 protein groups (unique peptide≥2) bound to the 5' region of IFV RNA including translation-related factors (16 ribosomal subunits, 3 eukaryotic initiation factor subunits, 1 elongation factor subunit and 6 potential internal ribosome entry site trans-acting factors), cytoskeleton-related proteins, membrane-related proteins, metabolism enzymes, and other proteins. These results can be used to study the translation and replication related factors of IFV interacting with host silkworm and to control flacherie disease in silkworm.
家蚕的空头病是由传染性空头病毒（IFV）引起的。IFV依靠其基因组RNA的5'区域招募宿主相关因子来进行病毒的翻译和复制。为了鉴定与IFV RNA的5'区域结合的宿主蛋白并确定对其功能重要的蛋白质，我们使用质谱法从家蚕中肠提取物中鉴定蛋白质，这些提取物是使用RNA适体标记的IFV RNA的5'区域获得的。我们发现325个蛋白质组（独特肽≥2）与IFV RNA的5'区域结合，包括翻译相关因子（16个核糖体亚基、3个真核起始因子亚基、1个延伸因子亚基和6个潜在的内部核糖体进入位点反式作用因子）、细胞骨架相关蛋白、膜相关蛋白、代谢酶和其他蛋白质。这些结果可用于研究IFV与宿主家蚕相互作用的翻译和复制相关因子，并用于控制家蚕的空头病。
