Semin B V
Genetika. 1989 Jun;25(6):981-92.
Full-size hybrid RNA-DNA molecules of mdg1, mdg3 and copia have been discovered in cultured cells of Drosophila. The intracellular distribution of these molecules is considered. Hybrid molecules are found both in the nuclei and cytoplasm, the latter comprising the major portion of molecules. The membrane subcellular fraction enriched by poly(A) RNA and poly(A) RNA-DNA molecules of retretransposons is isolated from cytoplasm. The full-size linear double-stranded DNA of mdg1 is found in the same fraction. The data obtained make it possible to suppose that the process of reverse transcription on the mdg RNA is the way of regulation of mdg expression in the cell.
在果蝇培养细胞中发现了mdg1、mdg3和copia的全长杂交RNA-DNA分子。对这些分子的细胞内分布进行了研究。杂交分子在细胞核和细胞质中均有发现，其中大部分位于细胞质中。从细胞质中分离出富含逆转座子的聚腺苷酸RNA和聚腺苷酸RNA-DNA分子的膜亚细胞组分。在同一组分中发现了mdg1的全长线性双链DNA。这些数据表明，mdg RNA的逆转录过程可能是细胞中mdg表达的调控方式。
