• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

果蝇移动分散遗传元件RNA的逆转录：中间形式的检测

Reverse transcription of Drosophila mobile dispersed genetic element RNAs: detection of intermediate forms.

作者信息

Arkhipova I R, Gorelova T V, Ilyin Y V, Schuppe N G

出版信息

Nucleic Acids Res. 1984 Oct 11;12(19):7533-48. doi: 10.1093/nar/12.19.7533.

DOI:10.1093/nar/12.19.7533
PMID:6208533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320179/
Abstract

DNA-RNA complexes were detected in cultured Drosophila melanogaster cells. These complexes are practically perfect hybrid molecules of mobile dispersed genes DNA (mdg1 and mdg3) and suitable poly(A)+RNA. Three species of single-stranded DNA have been found in hybrid molecules: full-sized mdg DNA containing either one or two LTRs (both minus strands) and single LTR sequences (plus strand). The properties of hybrid molecules are consistent with those being expected from the model of reverse transcription pathway of transposition and amplification of mdg elements.

摘要

在培养的黑腹果蝇细胞中检测到了DNA-RNA复合物。这些复合物实际上是移动分散基因DNA（mdg1和mdg3）与合适的聚腺苷酸加尾RNA（poly(A)+RNA）形成的完美杂交分子。在杂交分子中发现了三种单链DNA：含有一个或两个长末端重复序列（LTRs）的全长mdg DNA（两条负链）以及单个LTR序列（正链）。杂交分子的特性与mdg元件转座和扩增的逆转录途径模型所预期的特性一致。

相似文献

1
Reverse transcription of Drosophila mobile dispersed genetic element RNAs: detection of intermediate forms.果蝇移动分散遗传元件RNA的逆转录：中间形式的检测
Nucleic Acids Res. 1984 Oct 11;12(19):7533-48. doi: 10.1093/nar/12.19.7533.
2
[Detection of intermediates of RNA reverse transcription for mobile dispersed MDG 1 and MDG 3 genes in Drosophila cells].[果蝇细胞中移动分散的MDG 1和MDG 3基因RNA逆转录中间体的检测]
Mol Biol (Mosk). 1985 Jan-Feb;19(1):162-72.
3
Circular copies of mobile dispersed genetic elements in cultured Drosophila melanogaster cells.培养的黑腹果蝇细胞中移动分散遗传元件的环状拷贝。
Nucleic Acids Res. 1984 Oct 11;12(19):7517-31. doi: 10.1093/nar/12.19.7517.
4
The steps of reverse transcription of Drosophila mobile dispersed genetic elements and U3-R-U5 structure of their LTRs.果蝇移动分散遗传元件的逆转录步骤及其长末端重复序列（LTRs）的U3-R-U5结构。
Cell. 1986 Feb 28;44(4):555-63. doi: 10.1016/0092-8674(86)90265-5.
5
[Fine structure of long terminal repeats and stages of reverse transcription of mobile dispersed genes in Drosophila].[果蝇中长末端重复序列的精细结构及移动分散基因逆转录的阶段]
Genetika. 1986 Mar;22(3):378-89.
6
Mobile genetic elements in animal cells and their biological significance.动物细胞中的可移动遗传元件及其生物学意义。
Eur J Biochem. 1984 Dec 3;145(2):203-20. doi: 10.1111/j.1432-1033.1984.tb08541.x.
7
[Intracellular distribution of nucleotide sequences, homologous to mobile dispersed genes].[与移动分散基因同源的核苷酸序列的细胞内分布]
Genetika. 1989 Jun;25(6):981-92.
8
[Structure of long terminal repeats of transcriptionally active and inactive copies of the mobile dispersed gene MDG3 in Drosophila melanogaster].[黑腹果蝇中可移动分散基因MDG3转录活性和非活性拷贝的长末端重复序列结构]
Genetika. 1986 Mar;22(3):368-77.
9
Mobile dispersed genetic element MDG1 of Drosophila melanogaster: transcription pattern.黑腹果蝇的移动分散遗传元件MDG1：转录模式
Nucleic Acids Res. 1980 Nov 25;8(22):5347-61. doi: 10.1093/nar/8.22.5347.
10
Extrachromosomal DNA forms of copia-like transposable elements, F elements and middle repetitive DNA sequences in Drosophila melanogaster. Variation in cultured cells and embryos.黑腹果蝇中类copia转座元件、F元件的染色体外DNA形式及中度重复DNA序列。培养细胞和胚胎中的变异。
J Mol Biol. 1985 Mar 5;182(1):31-43. doi: 10.1016/0022-2836(85)90025-7.

引用本文的文献

1
The nature of unstable insertion mutations and reversions in the locus cut of Drosophila melanogaster: molecular mechanism of transposition memory.果蝇黑素体切割基因座位中不稳定插入突变和回复突变的本质：转座记忆的分子机制。
EMBO J. 1985 Dec 30;4(13B):3781-7. doi: 10.1002/j.1460-2075.1985.tb04148.x.
2
Complex patterns of transcription of a Drosophila retrotransposon in vivo and in vitro by RNA polymerases II and III.果蝇反转录转座子在体内外由RNA聚合酶II和III进行转录的复杂模式。
Nucleic Acids Res. 1995 Nov 11;23(21):4480-7. doi: 10.1093/nar/23.21.4480.
3
Illegitimate recombination in the histone multigenic family generates circular DNAs in Drosophila embryos.

本文引用的文献

1
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.果蝇乙醇脱氢酶基因的分离与部分特性分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8. doi: 10.1073/pnas.77.10.5794.
2
The origin of extrachromosomal circular copia elements.染色体外环状考皮亚元件的起源。
Cell. 1983 Sep;34(2):415-9. doi: 10.1016/0092-8674(83)90375-6.
3
Structure of extrachromosomal circular DNAs containing both the Alu family of dispersed repetitive sequences and other regions of chromosomal DNA.包含分散重复序列的Alu家族和染色体DNA其他区域的染色体外环状DNA的结构。
组蛋白多基因家族中的非法重组在果蝇胚胎中产生环状DNA。
Nucleic Acids Res. 1988 Sep 26;16(18):8817-33. doi: 10.1093/nar/16.18.8817.
4
Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells.多种机制在中国仓鼠卵巢细胞中产生染色体外环状DNA。
Nucleic Acids Res. 1986 Apr 25;14(8):3527-38. doi: 10.1093/nar/14.8.3527.
5
The termini of extrachromosomal linear copia elements.染色体外线性考皮阿元件的末端。
Nucleic Acids Res. 1986 May 12;14(9):3659-69. doi: 10.1093/nar/14.9.3659.
6
Identification and nucleotide sequence determination of a potential primer tRNA for reverse transcription of a Drosophila retrotransposon, 297.果蝇反转录转座子297逆转录潜在引物tRNA的鉴定及核苷酸序列测定
Nucleic Acids Res. 1986 Apr 11;14(7):3031-43. doi: 10.1093/nar/14.7.3031.
7
Identification of genes for reverse transcriptase-like enzymes in two Drosophila retrotransposons, 412 and gypsy; a rapid detection method of reverse transcriptase genes using YXDD box probes.在两种果蝇逆转录转座子412和gypsy中鉴定类逆转录酶基因；一种使用YXDD盒探针快速检测逆转录酶基因的方法。
Nucleic Acids Res. 1986 Apr 11;14(7):3017-30. doi: 10.1093/nar/14.7.3017.
8
Activation of putative transposition intermediate formation in tumor cells.肿瘤细胞中假定转座中间体形成的激活。
EMBO J. 1985 Sep;4(9):2209-15. doi: 10.1002/j.1460-2075.1985.tb03916.x.
9
Characterization of products of TY1-mediated reverse transcription in Saccharomyces cerevisiae.酿酒酵母中TY1介导的逆转录产物的特征分析。
Mol Gen Genet. 1991 Apr;226(1-2):145-53. doi: 10.1007/BF00273598.
10
Leader region of mdg1 Drosophila retrotransposon RNA contains 3'-end processing sites.果蝇反转录转座子mdg1 RNA的引导区域包含3'末端加工位点。
Nucleic Acids Res. 1991 Jun 25;19(12):3213-9. doi: 10.1093/nar/19.12.3213.
J Mol Biol. 1984 Mar 25;174(1):41-54. doi: 10.1016/0022-2836(84)90364-4.
4
Some extrachromosomal circular DNAs containing the Alu family of dispersed repetitive sequences may be reverse transcripts.一些含有分散重复序列的Alu家族的染色体外环状DNA可能是逆转录产物。
J Mol Biol. 1984 Mar 25;174(1):31-40. doi: 10.1016/0022-2836(84)90363-2.
5
Close relationship between the long terminal repeats of avian leukosis-sarcoma virus and copia-like movable genetic elements of Drosophila.禽白血病-肉瘤病毒的长末端重复序列与果蝇类copia可移动遗传元件之间的密切关系。
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3193-7. doi: 10.1073/pnas.80.11.3193.
6
Retroviruses and transposable elements--which came first?逆转录病毒和转座因子——哪个先出现？
Nature. 1983 Mar 10;302(5904):105-6. doi: 10.1038/302105a0.
7
Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells.培养的果蝇细胞中真核转座因子copia的染色体外环状拷贝。
Nature. 1981 Aug 13;292(5824):591-5. doi: 10.1038/292591a0.
8
Isolation and characterization of a new family of mobile dispersed genetic elements, mdg3, in Drosophila melanogaster.黑腹果蝇中一个新的可移动分散遗传元件家族mdg3的分离与鉴定。
Chromosoma. 1980;81(1):27-53. doi: 10.1007/BF00292421.
9
RNA viruses. Reverse transcription in plants?RNA病毒。植物中的逆转录？
Nature. 1983;304(5922):116-7. doi: 10.1038/304116a0.
10
RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.来自酵母转座因子Ty1的RNA在直接重复序列中都有两端，这种结构类似于逆转录病毒RNA。
Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6. doi: 10.1073/pnas.80.9.2432.