Mukhamedshin E K, Tarasov V A
Genetika. 1989 Jul;25(7):1160-7.
HfeTn5-(04,06) and IfeTn9 mutations increase efficiency of precise excision of Tn5, Tn10 and decrease that of Tn9. These mutations have been mapped in uvrD gene. In LFETn9 and UVRE502 mutants, the multicopy plasmid pEM61 carrying the cloned uvrD gene complements LFETn9- phenotype (Low Frequency Excision of Tn9). These results indicate that the uvrD gene product plays different role in excision of transposons with long and short inverted repeats. The mechanism of this effect is discussed.
HfeTn5-(04,06)和IfeTn9突变提高了Tn5、Tn10精确切除的效率,降低了Tn9的精确切除效率。这些突变已定位在uvrD基因中。在LFETn9和UVRE502突变体中,携带克隆uvrD基因的多拷贝质粒pEM61可互补LFETn9-表型(Tn9低频切除)。这些结果表明,uvrD基因产物在具有长和短反向重复序列的转座子切除中发挥不同作用。讨论了这种效应的机制。