[recB和recC基因突变对Tn5从pNM1质粒基因组中精确切除的影响]
[The effect of mutations in recB and recC genes on the precise excision of Tn5 from pNM1 plasmid genome].
作者信息
Davenis S V, Markov A P, Golubev A V, Smirnov G B
出版信息
Mol Gen Mikrobiol Virusol. 1988 Sep(9):14-7.
PMID:2850499
Abstract
The affect of mutations in chromosomal genes determining the realization of RecBC and RecF pathways of recombination in E. coli K12 on the frequency of transposon Tn5 precise excision from the genome of the conjugative plasmid pNM1 has been demonstrated. The pNM1 plasmid is a derivative of R100.1 and differs from the latter in the presence of Tn5 inactivating the tet gene of transposon Tn10.
摘要
已证明，大肠杆菌K12中决定RecBC和RecF重组途径实现的染色体基因突变对转座子Tn5从接合质粒pNM1基因组中精确切除频率的影响。pNM1质粒是R100.1的衍生物，与后者的不同之处在于存在使转座子Tn10的tet基因失活的Tn5。
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