Zanocco A L, Pavez R, Videla L A, Lissi E A
Organic Chemistry Department, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago.
Free Radic Biol Med. 1989;7(2):151-6. doi: 10.1016/0891-5849(89)90006-3.
2,2'-Azobis-[2-amidinopropane] initiated lipid peroxidation of egg yolk phosphatydil choline liposomes was measured by oxygen uptake and the emitted visible luminescence. Lipid peroxidation involved a chain process (kinetic chain length = 49 +/- 11) and its rate was independent of added Fe ions. Diethyldithiocarbamate (DDC) behaved as an efficient inhibitor in the microM range, being able to trap 1.05 +/- 0.25 free radicals per added molecule. The efficiency of DDC was also independent of Fe addition to the system. These results indicate that DDC is able to trap the chain carrying free radicals, showing that this compound, besides being a powerful metal chelator, is also an efficient free radical scavenger. It is proposed that the relevant step in this process involves an electron transfer from DDC to the peroxy radical LOO., LOO. + DDC- ----LOO- + DDC. followed by protonation of LOO- and dimerization of the DDC. radical.
通过氧摄取和发射的可见发光来测量2,2'-偶氮双-[2-脒基丙烷]引发的蛋黄磷脂酰胆碱脂质体的脂质过氧化。脂质过氧化涉及一个链式过程（动力学链长 = 49 ± 11），其速率与添加的铁离子无关。二乙基二硫代氨基甲酸盐（DDC）在微摩尔范围内表现为一种有效的抑制剂，每添加一个分子能够捕获1.05 ± 0.25个自由基。DDC的效率也与向系统中添加铁无关。这些结果表明DDC能够捕获携带自由基的链，表明该化合物除了是一种强大的金属螯合剂外，也是一种有效的自由基清除剂。有人提出该过程中的相关步骤涉及电子从DDC转移到过氧自由基LOO·，LOO· + DDC- ----LOO- + DDC。随后LOO-质子化和DDC自由基二聚化。
