Olek Robert Antoni, Ziolkowski Wieslaw, Kaczor Jan Jacek, Greci Lucedio, Popinigis Jerzy, Antosiewicz Jedrzej
Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport, Wiejska 1, 80-336 Gdansk, Poland.
J Biochem Mol Biol. 2004 Jul 31;37(4):416-21. doi: 10.5483/bmbrep.2004.37.4.416.
The antioxidant activities of NADH and of its analogue, 1,4-dihydro-2,6-dimethyl-3,5-dicarbethoxy-pyridine (PyH(2)), were evaluated in vitro. NADH was found to be oxidized by the peroxyl radical derived from 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH) decomposition, in a pH-dependent manner. Both NADH and PyH(2) inhibited the peroxidation of egg yolk lecithin (EYL) liposomes, although PyH(2) was more effective than NADH when 2,2'-azobis-4-methoxy-2,4-dimethyl-valeronitrile (methoxy-AMVN) was employed to induce EYL liposome peroxidation. The antioxidant activities of NADH and PyH(2) were also evaluated by measuring their influences on 1,3-diphenylisobenzofuran (DPBF) fluorescence decay in the presence of peroxyl radicals. NADH and PyH(2) were much more effective at inhibiting DPBF quenching in Triton X-100 micelles than in liposomes. These results indicate that NADH can inhibit lipid peroxidation despite being hydrophilic. Nevertheless, membrane penetration is an important factor and limits its antioxidant activity.
在体外评估了烟酰胺腺嘌呤二核苷酸（NADH）及其类似物1,4 - 二氢 - 2,6 - 二甲基 - 3,5 - 二乙氧羰基吡啶（PyH(2)）的抗氧化活性。发现NADH会被2,2 - 偶氮二（2 - 脒基丙烷）二盐酸盐（AAPH）分解产生的过氧自由基以pH依赖的方式氧化。NADH和PyH(2)均能抑制蛋黄卵磷脂（EYL）脂质体的过氧化，不过当使用2,2'-偶氮二（4 - 甲氧基 - 2,4 - 二甲基戊腈）（甲氧基 - AMVN）诱导EYL脂质体过氧化时，PyH(2)比NADH更有效。还通过测量NADH和PyH(2)在过氧自由基存在下对1,3 - 二苯基异苯并呋喃（DPBF）荧光衰减的影响来评估它们的抗氧化活性。在Triton X - 100胶束中，NADH和PyH(2)在抑制DPBF猝灭方面比在脂质体中更有效。这些结果表明，尽管NADH具有亲水性，但它仍能抑制脂质过氧化。然而，膜穿透是一个重要因素，会限制其抗氧化活性。
