Rodríguez E, Pickard M A, Vazquez-Duhalt R
Instituto de Biotecnología UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62250 México.
Curr Microbiol. 1999 Jan;38(1):27-32. doi: 10.1007/pl00006767.
White-rot fungi were studied for the decolorization of 23 industrial dyes. Laccase, manganese peroxidase, lignin peroxidase, and aryl alcohol oxidase activities were determined in crude extracts from solid-state cultures of 16 different fungal strains grown on whole oats. All Pleurotus ostreatus strains exhibited high laccase and manganese peroxidase activity, but highest laccase volumetric activity was found in Trametes hispida. Solid-state culture on whole oats showed higher laccase and manganese peroxidase activities compared with growth in a complex liquid medium. Only laccase activity correlated with the decolorization activity of the crude extracts. Two laccase isoenzymes from Trametes hispida were purified, and their decolorization activity was characterized.
对白腐真菌进行了研究，以考察其对23种工业染料的脱色能力。测定了16种不同真菌菌株在全燕麦上进行固态培养所得粗提物中的漆酶、锰过氧化物酶、木质素过氧化物酶和芳醇氧化酶活性。所有平菇菌株均表现出较高的漆酶和锰过氧化物酶活性，但糙皮侧耳的漆酶体积活性最高。与在复合液体培养基中生长相比，在全燕麦上进行固态培养显示出更高的漆酶和锰过氧化物酶活性。只有漆酶活性与粗提物的脱色活性相关。对糙皮侧耳的两种漆酶同工酶进行了纯化，并对其脱色活性进行了表征。
