Kumari B, Rajput S, Gaur P, Singh S N, Singh D P
Babasaheb Bhimrao Ambedkar University School of Environmental Science Lucknow India.
CSIR—NBRI Plant Ecology and Environmental Science Lucknow India.
Cell Mol Biol (Noisy-le-grand). 2014 Dec 24;60(5):22-8.
High molecular weight poly aromatic hydrocarbons (HMW PAHs) are well known for their hydrophobicity and they get strongly adsorbed onto the soil particles. Generally, surfactants facilitate the biodegradation of PAH by enhancing their solubility and desorption of hydrophobic compounds from soil particles. To investigate the role of synthetic surfactant in biodegradation of PAHs, two bacterial strains BP10 and P2 were incubated in soil spiked with pyrene and phenantherene (100 μg g-1of soil each) in isolation and in combination with/without Tween 80. After 14 days of incubation, pyrene and phenantherene were degraded by a combination of BP10 and P2 to the extent of 98% and 99%, respectively. Addition of tween 80 reduced the degradation of pyrene and phenantherene by 35 and 10%, respectively. Biosurfactant produced by selected strains i.e. BP10 and P2 could enhance desorption of pyrene (100 μg g-1of soil) by about 27% and 12%, respectively. However, desorption activity was relatively higher (32 and 29%, respectively) in case of phenanthrene (100 μg g-1of soil) from the spiked soil. Present study showed that in spite of additional chemical surfactant, bioaugmentation of highly petroleum hydrocarbon degrading bacterial combination was very effective in boosting the bioremediation of PAHs- contaminated sites.
高分子量多环芳烃（HMW PAHs）以其疏水性而闻名，它们会强烈吸附在土壤颗粒上。一般来说，表面活性剂通过提高多环芳烃的溶解度以及促进疏水性化合物从土壤颗粒上的解吸来促进其生物降解。为了研究合成表面活性剂在多环芳烃生物降解中的作用，将两种细菌菌株BP10和P2分别单独培养，以及在添加/不添加吐温80的情况下，与掺入芘和菲（每克土壤各100μg）的土壤一起培养。培养14天后，BP10和P2的组合分别将芘和菲降解了98%和99%。添加吐温80分别使芘和菲的降解率降低了35%和10%。所选菌株即BP10和P2产生的生物表面活性剂可分别使芘（每克土壤100μg）的解吸率提高约27%和12%。然而，对于掺入菲（每克土壤100μg）的土壤，解吸活性相对较高（分别为32%和29%）。目前的研究表明，尽管添加了化学表面活性剂，但高度降解石油烃的细菌组合进行生物强化在促进多环芳烃污染场地的生物修复方面非常有效。
