Chang Jae-Soo, Cha Daniel K, Radosevich Mark, Jin Yan
a Department of Environmental Engineering , Korea Maritime and Ocean University , Busan , Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(6):611-6. doi: 10.1080/10934529.2015.994967.
This study investigated the effects of surfactant-producing microorganism, Pseudomonas aeruginosa ATCC 9027, on phenanthrene (PHE) biodegradation by two different PHE-degrading bacteria (Isolate P5-2 and Pseudomonas strain R) in soil. Phenanthrene mineralization experiments were conducted with soils inoculated with one of PHE-degraders and/or the surfactant-producer. Influence of co-inoculation with the surfactant-producing bacteria on phenanthrene transport and biodegradation was also examined in soil columns. P. strain R mineralized phenanthrene faster and to a greater extent than Isolate P5-2 in the test soil. Co-inoculation with the surfactant-producing bacteria significantly enhanced phenanthrene biodegradation by P. strain R but it did not affect the biodegradation by Isolate P5-2 in both batch and column systems. Production of biosurfactants by P. aeruginosa ATCC 9027 was negligible under the given conditions. This study demonstrated that bioaugmentation with surfactant-producing bacteria could enhance in situ bioremediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs) and the beneficial effect of the bioaugmentation depended on types of PAH-degrading microorganisms present.
本研究调查了产表面活性剂微生物铜绿假单胞菌ATCC 9027对两种不同的菲（PHE）降解细菌（分离株P5-2和假单胞菌菌株R）在土壤中对菲（PHE）生物降解的影响。用接种了一种PHE降解菌和/或产表面活性剂菌的土壤进行了菲矿化实验。还在土柱中研究了与产表面活性剂细菌共接种对菲迁移和生物降解的影响。在试验土壤中，菌株R比分离株P5-2更快且更大量地矿化菲。在分批和柱系统中，与产表面活性剂细菌共接种显著增强了菌株R对菲的生物降解，但对分离株P5-2的生物降解没有影响。在给定条件下，铜绿假单胞菌ATCC 9027产生的生物表面活性剂可忽略不计。本研究表明，用产表面活性剂细菌进行生物强化可增强对多环芳烃（PAHs）污染土壤的原位生物修复，且生物强化的有益效果取决于存在的PAH降解微生物的类型。
