Eom Tae-Yeon, Stanco Amelia, Guo Jiami, Wilkins Gary, Deslauriers Danielle, Yan Jessica, Monckton Chase, Blair Joshua, Oon Eesim, Perez Abby, Salas Eduardo, Oh Adrianna, Ghukasyan Vladimir, Snider William D, Rubenstein John L R, Anton E S
UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Department of Psychiatry, Neuroscience Program, and Nina Ireland Laboratory of Developmental Neurobiology, University of California, San Francisco, San Francisco, CA 94158-2324, USA.
Dev Cell. 2014 Dec 22;31(6):677-89. doi: 10.1016/j.devcel.2014.11.022.
Coordinated migration of distinct classes of neurons to appropriate positions leads to the formation of functional neuronal circuitry in the cerebral cortex. The two major classes of cortical neurons, interneurons and projection neurons, utilize distinctly different modes (radial versus tangential) and routes of migration to arrive at their final positions in the cerebral cortex. Here, we show that adenomatous polyposis coli (APC) modulates microtubule (MT) severing in interneurons to facilitate tangential mode of interneuron migration, but not the glial-guided, radial migration of projection neurons. APC regulates the stability and activity of the MT-severing protein p60-katanin in interneurons to promote the rapid remodeling of neuronal processes necessary for interneuron migration. These findings reveal how severing and restructuring of MTs facilitate distinct modes of neuronal migration necessary for laminar organization of neurons in the developing cerebral cortex.
不同类型神经元向合适位置的协调迁移导致大脑皮质中功能性神经回路的形成。皮质神经元的两大类，即中间神经元和投射神经元，利用截然不同的迁移模式（径向与切向）和途径到达它们在大脑皮质中的最终位置。在这里，我们表明腺瘤性息肉病 coli（APC）调节中间神经元中的微管（MT）切断，以促进中间神经元的切向迁移模式，但不影响投射神经元由胶质细胞引导的径向迁移。APC 调节中间神经元中 MT 切断蛋白 p60-卡坦宁的稳定性和活性，以促进中间神经元迁移所需的神经元突起的快速重塑。这些发现揭示了 MT 的切断和重组如何促进发育中的大脑皮质中神经元分层组织所需的不同神经元迁移模式。
