Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
Dev Cell. 2014 Dec 22;31(6):774-83. doi: 10.1016/j.devcel.2014.11.003.
Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.
Kupffer's 囊 (KV) 是鱼类左右对称的器官，沿胚胎的左右 (LR) 轴进行模式化。腔衬上皮细胞内的细胞形状的区域差异对于其 LR 模式化功能至关重要。然而，KV 细胞获得其特征形状的过程在很大程度上是未知的。在这里，我们表明，脊索通过触发腔衬上皮细胞的腔旁前背 (AD) 区域的细胞外基质 (ECM) 积累，诱导 KV 内的细胞形状的区域差异。这种局部 ECM 沉积限制了腔生长过程中腔衬上皮细胞在 KV 的 AD 区域的顶端扩张。我们的研究为 KV 将全局胚胎模式化转化为其 LR 对称破缺功能所需的区域细胞形状差异的过程提供了机制上的见解。
