Manna Raj Kumar, Retzlaff Emma M, Hinman Anna Maria, Lan Yiling, Abdel-Razek Osama, Bates Mike, Hehnly Heidi, Amack Jeffrey D, Manning M Lisa
Department of Physics Syracuse University, Syracuse, NY 13244, USA.
BioInspired Institute, Syracuse University, Syracuse, NY 13244, USA.
bioRxiv. 2024 Jul 17:2024.07.13.603371. doi: 10.1101/2024.07.13.603371.
Organs and tissues must change shape in precise ways during embryonic development to execute their functions. Multiple mechanisms including biochemical signaling pathways and biophysical forces help drive these morphology changes, but it has been difficult to tease apart their contributions, especially from tissue-scale dynamic forces that are typically ignored. We use a combination of mathematical models and experiments to study a simple organ in the zebrafish embryo called Kupffer's vesicle. Modeling indicates that dynamic forces generated by tissue movements in the embryo produce shape changes in Kupffer's vesicle that are observed during development. Laser ablations in the zebrafish embryo that alter these forces result in altered organ shapes matching model predictions. These results demonstrate that dynamic forces sculpt organ shape during embryo development.
在胚胎发育过程中，器官和组织必须以精确的方式改变形状才能执行其功能。包括生化信号通路和生物物理力在内的多种机制有助于推动这些形态变化，但很难区分它们各自的作用，尤其是那些通常被忽视的组织尺度动态力的作用。我们结合数学模型和实验，研究斑马鱼胚胎中一个名为库普弗囊泡的简单器官。建模结果表明，胚胎中组织运动产生的动态力会使库普弗囊泡产生发育过程中观察到的形状变化。在斑马鱼胚胎中进行的激光消融改变了这些力，导致器官形状改变，与模型预测相符。这些结果表明，动态力在胚胎发育过程中塑造了器官的形状。
