利用靶向敲除突变体集合进行果蝇 microRNA 功能的系统研究。

Systematic study of Drosophila microRNA functions using a collection of targeted knockout mutations.

机构信息

Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673, Singapore.

Procter and Gamble International Operations SA Singapore Branch, Quantitative Sciences, Statistics Asia, 70 Biopolis Street, Singapore 138547, Singapore.

出版信息

Dev Cell. 2014 Dec 22;31(6):784-800. doi: 10.1016/j.devcel.2014.11.029.

DOI:10.1016/j.devcel.2014.11.029

PMID:25535920

Abstract

MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

摘要

微 RNA 广泛存在于动物基因组中，但对于它们在体内的功能却知之甚少。在这里，我们通过靶向同源重组产生了 80 个新的果蝇微 RNA 突变体。这些突变体去除了 104 个微 RNA。加上之前报道的 15 个突变体，这个集合包括 15 个突变体，删除了 130 个微 RNA。总的来说，这些基因产生了超过 99%的果蝇微 RNA，通过微 RNA 序列读取来衡量。我们对发育和成年微 RNA 表型进行了调查。超过 80%的突变体在使用 p < 0.01 显著性阈值时表现出至少一种表型。我们观察到微 RNA 丰度与与生存和寿命相关的表型之间存在显著相关性，但与大多数其他表型无关。微 RNA 簇突变体产生显著表型的可能性不比单个微 RNA 突变体高。这个突变体集合将为未来分析果蝇微 RNA 的生物学功能提供资源。