Sokolova T M, Shuvalov A N, Poloskov V V, Shapoval I M, Kostinov M P
Zh Mikrobiol Epidemiol Immunobiol. 2014 Sep-Oct(5):37-43.
Study the effect of inactivated influenza vaccines on the activity of innate and adaptive immunity genes (TLR3, TLR4 and B2M), RNA-interference Dicer1-gene, production of cytokines (antiviral IFN type I and II, regulatory IL10, IL17) and pro-inflammatory factors IL1-β, TNFα.
Gene expression was determined by rRT-PCR with authors' primers in human blood cells treated with various doses of the vaccines. Concentration of cytokines by enzyme immunoassay was measured in cultural fluid using "Vector-best" kits.
The studied vaccines have characteristic effects on genetic level. Grippol vaccine predominately stimulates TLR4 gene, activates TLR3, B2M and Dicer1 genes. Influvac vaccine mostly induces TLR3 gene and to a lesser extent TLR4 gene, does not influence the expression of B2M gene and inhibits Dicer1 gene. Vaxigrip split vaccine--the most potent stimulator of gene activity at low doses. Its main targets are TLR3 and B2M genes. All the inactivated vaccines--inductors of high level of IFNγ, low level of TNFα and do not induce IL17. Grippol additionally stimulates secretion of IL1-β, and Vaxigrip - IFNα. Subunit vaccines Grippol and Influvac that contain purified influenza virus hemagglutinins induce IL10 synthesis in blood cells.
Immunogenetic characteristics of the inactivated influenza vaccines administered nowadays are obtained.
研究灭活流感疫苗对固有免疫和适应性免疫基因（TLR3、TLR4和B2M）、RNA干扰Dicer1基因的活性、细胞因子（抗病毒I型和II型干扰素、调节性IL10、IL17）以及促炎因子IL1-β、TNFα产生的影响。
采用作者自行设计的引物，通过逆转录-实时荧光定量聚合酶链反应（rRT-PCR）测定不同剂量疫苗处理后人血细胞中的基因表达。使用“Vector-best”试剂盒，通过酶免疫测定法检测培养液中细胞因子的浓度。
所研究的疫苗在基因水平上具有特征性作用。Grippol疫苗主要刺激TLR4基因，激活TLR3、B2M和Dicer1基因。Influvac疫苗主要诱导TLR3基因，对TLR4基因的诱导作用较小，不影响B2M基因的表达，并抑制Dicer1基因。Vaxigrip裂解疫苗是低剂量时最有效的基因活性刺激剂。其主要作用靶点是TLR3和B2M基因。所有灭活疫苗都是高水平IFNγ、低水平TNFα的诱导剂，且不诱导IL17。Grippol疫苗还能刺激IL1-β的分泌，Vaxigrip疫苗则刺激IFNα的分泌。含有纯化流感病毒血凝素的亚单位疫苗Grippol和Influvac可诱导血细胞中IL10的合成。
获得了目前使用的灭活流感疫苗的免疫遗传学特征。
