Chromova E A, Akhmatova E A, Skhodova S A, Semochkin L A, Khomenkov V G, Akhmatova N K, Kostinov M P
Zh Mikrobiol Epidemiol Immunobiol. 2016 Sep(5):23-28.
Study the effect of Vaxigrip split, Influvac subunit and Grippol plus immune-adjuvanted vaccines on the content of myeloid (mDC) and plasmacytoid (pDC) dendritic cells (DC) in blood of vaccinated healthy women. Materials andmethods. Blood of 30 healthy women aged 18-50 years was studied at days 7 and 30 after the vaccination. pDC (CD14+CD16-/CD85k(ILT3)-PE/ CD123-PC5) and mDC (CD14+CD-16-/CD85k(ILT3)-PE/CD33 -PC5) immune phenotyping was carried out using mAbs (Beckman Coulter, France) and flow cytometer Cytomix FC-500 (Beckman Coulter, USA).
Use of unadjuvanted vaccines Vaxigrip and Influvac resulted in an increase of the numbers of mDC and pDC (p<0.05) in blood of the vaccinated only at day 7 of the observation. Grippol resulted in a more significant (2.2 - 3.6 times, p<0.05) increase of DC subpopulations (compared with unadjuvanted vaccines) at both day 7 and a month after the vaccination.
Influenza vaccination activated innate effectors - the first component on the way of infection penetration - dendritic cells of both myeloid and lymphoid origin. Wherein, a more pronounced and prolonged effect of such activation is observed when immune-adjuvanted vaccine is used compared with subunit and split vaccines.
研究裂解流感疫苗（Vaxigrip）、亚单位流感疫苗（Influvac）和加佐剂流感疫苗（Grippol plus）对接种疫苗的健康女性血液中髓样（mDC）和浆细胞样（pDC）树突状细胞（DC）含量的影响。材料与方法。对30名年龄在18 - 50岁的健康女性在接种疫苗后第7天和第30天的血液进行研究。使用单克隆抗体（法国贝克曼库尔特公司）和流式细胞仪Cytomix FC - 500（美国贝克曼库尔特公司）对pDC（CD14 + CD16 - /CD85k(ILT3) - PE/CD123 - PC5）和mDC（CD14 + CD - 16 - /CD85k(ILT3) - PE/CD33 - PC5）进行免疫表型分析。
使用无佐剂疫苗Vaxigrip和Influvac仅在观察第7天时导致接种者血液中mDC和pDC数量增加（p<0.05）。Grippol在接种疫苗后第7天和第30天均导致DC亚群数量更显著增加（比无佐剂疫苗增加2.2 - 3.6倍，p<0.05）。
流感疫苗接种激活了天然效应细胞——感染侵入途径的第一组成部分——髓样和淋巴样来源的树突状细胞。其中，与亚单位疫苗和裂解疫苗相比，使用加佐剂疫苗时观察到这种激活作用更明显且持续时间更长。
