原发性胃黏膜相关淋巴组织淋巴瘤中不存在或罕见涉及p65的BCL10畸变和核因子-κB激活。

BCL10 aberations and NF-kappa B activation involving p65 are absent or rare in primary gastric MALT lymphoma.

作者信息

Hajder Jelena, Marisavljević Dragomir, Stanisavljević Natasa, Mihaljević Biljana, Kovcin Vladimir, Marković Olivera, Zivković Radmila

出版信息

Vojnosanit Pregl. 2014 Nov;71(11):1040-4. doi: 10.2298/vsp130813035h.

DOI:10.2298/vsp130813035h

PMID:25536807

Abstract

BACKGROUND/AIM: Mucosa-associated lymphoid tissue (MALT) lymphoma accounts for 5-17% non-Hodgkin lymphomas (NHL). The molecular pathogenesis of MALT lymphomas is not well-established. The aim of this study was to evaluate immunohistochemically determined nuclear coexpression of BCL10 and NF-kappaB (NF-kappaB) in tumor cells of gastric MALT lymphoma and its impact on the patogenesis and outcome of the disease.

METHODS

Medical records of 35 patients with newly diagnosed gastric MALT lymphoma were analyzed and biopsy specimens were immunostained for BCL10 and NF-kappaB expression (p65 subunit).

RESULTS

The median age of 35 patients diagnosed with gastric MALT lymphoma was 63.5 years (male/female = 21/14). Symptoms were present in 23/35 (65.7%) patients with the weight loss as the most common symptom. Gastric MALT lymphomas were usually localized in the stomach corpus and corpus and antrum (45.7% and 31.2%, respectively). H. pylon infection was confirmed in 20 out of 30 (66.7%) patients. Treatment options were as follows: immunochemotherapy in 10 (28.5%) patients, surgery in 9 (25.8%) patients, combined surgery and chemotherapy in 14 (40%) patients and supportive measures in 2 (5.7%) patients. Complete remission was achieved in 13 (37.10/) patients and partial remission in two (5.7%/) patients. Sixteen (45.7%/) patients had disease progression (p < 0.001). Cytoplasmatic expression of BCL10 in tumor cells was detected in 19 (54.3%) specimens. Nuclear expression was detected in no specimen. Cytoplasmic expression of NF-kappaB was present in 22 (65.7%) specimens, but nuclear expression was not detected in any specimens.

CONCLUSION

Nuclear expressions (activation)of NF-kappaB p65 subunit and BCL10 were not detected in specimens of gastric MALT lymphoma. The correlation of nuclear coexpression of BCL10 and NF-kappaB in gastric MALT lymphoma was not established. These results indicate that other mechanisms and signal pathways are active in lymphogenesis of gastric MALT lymphoma, as that apoptotic inhibition is not the main, nor the only mechanism in tumorogenesis.

摘要

背景/目的：黏膜相关淋巴组织（MALT）淋巴瘤占非霍奇金淋巴瘤（NHL）的5%-17%。MALT淋巴瘤的分子发病机制尚未完全明确。本研究旨在通过免疫组化评估胃MALT淋巴瘤肿瘤细胞中BCL10和核因子κB（NF-κB）的核共表达情况及其对疾病发病机制和预后的影响。

方法

分析35例新诊断的胃MALT淋巴瘤患者的病历，并对活检标本进行BCL10和NF-κB表达（p65亚基）的免疫染色。

结果

35例诊断为胃MALT淋巴瘤的患者中位年龄为63.5岁（男/女=21/14）。23/35（65.7%）的患者有症状，其中体重减轻是最常见的症状。胃MALT淋巴瘤通常位于胃体部以及胃体和胃窦部（分别为45.7%和31.2%）。30例患者中有20例（66.7%）确诊有幽门螺杆菌感染。治疗方案如下：10例（28.5%）患者接受免疫化疗，9例（25.8%）患者接受手术治疗，14例（40%）患者接受手术联合化疗，2例（5.7%）患者采取支持措施。13例（37.1%）患者达到完全缓解，2例（5.7%）患者部分缓解。16例（45.7%）患者疾病进展（p<0.001）。19份（54.3%）标本检测到肿瘤细胞中BCL10的细胞质表达，未在任何标本中检测到核表达。22份（65.7%）标本存在NF-κB的细胞质表达，但未在任何标本中检测到核表达。