Meredith Tricia L, Corcoran Alan, Roper Stephen D
Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, 1600 NW 10th Avenue, Miami, FL 33136, USA and Present address: Department of Biological Sciences, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431, USA
Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, 1600 NW 10th Avenue, Miami, FL 33136, USA and.
Chem Senses. 2015 May;40(4):217-22. doi: 10.1093/chemse/bju066. Epub 2014 Dec 23.
Leptin, a peptide hormone released by adipose tissue, acts on the hypothalamus to control cravings and appetite. Leptin also acts to decrease taste responses to sweet substances, though there is little detailed information regarding where leptin acts in the taste transduction cascade. The present study examined the effects of leptin on sweet-evoked responses and neuro transmitter release from isolated taste buds. Our results indicate that leptin moderately decreased sweet-evoked calcium mobilization in isolated mouse taste buds. We also employed Chinese hamster ovary biosensor cells to examine taste transmitter release from isolated taste buds. Leptin reduced ATP and increased serotonin release in response to sweet stimulation. However, leptin has no effect on bitter-evoked transmitter release, further showing that the action of leptin is sweet specific. Our results support those of previous studies, which state that leptin acts on taste tissue via the leptin receptor, most likely on Type II (Receptor) cells, but also possibly on Type III (Presynaptic) cells.
瘦素是一种由脂肪组织释放的肽类激素，作用于下丘脑以控制渴望和食欲。瘦素还能降低对甜味物质的味觉反应，不过关于瘦素在味觉转导级联反应中的作用位点，详细信息较少。本研究检测了瘦素对分离出的味蕾的甜味诱发反应和神经递质释放的影响。我们的结果表明，瘦素适度降低了分离出的小鼠味蕾中甜味诱发的钙动员。我们还利用中国仓鼠卵巢生物传感器细胞来检测分离出的味蕾的味觉递质释放。瘦素在甜味刺激下减少了三磷酸腺苷（ATP）的释放并增加了血清素的释放。然而，瘦素对苦味诱发的递质释放没有影响，进一步表明瘦素的作用具有甜味特异性。我们的结果支持了先前的研究，这些研究表明瘦素通过瘦素受体作用于味觉组织，最有可能作用于II型（受体）细胞，但也可能作用于III型（突触前）细胞。
