Department of Medical Research, China Medical University Hospital, No. 91 Hsueh-Shih Road, Taichung 402, Taiwan; Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
Bioresour Technol. 2015 May;184:2-8. doi: 10.1016/j.biortech.2014.11.097. Epub 2014 Dec 3.
The algal β-carotene hydroxylase gene Crchyb from Chlamydomonas reinhardtii, Czchyb from Chlorella zofingiensis, or Hpchyb from Haematococcus pluvialis and six other carotenoid-synthesis pathway genes were co-integrated into the genome of a yeast host. Each of these three algal genes showed a higher efficiency to convert β-carotene to downstream carotenoids than the fungal genes from Phaffia rhodozyma. Furthermore, the strain with Hpchyb displayed a higher carotenoid productivity than the strains integrated with Crchyb or Czchyb, indicating that Hpchyb is more efficient than Crchyb and Czchyb. These results suggest that β-carotene hydroxylase plays a crucial role in the biosynthesis of carotenoids.
藻类β-胡萝卜素羟化酶基因 Crchyb 来自莱茵衣藻、Czchyb 来自栅藻或 Hpchyb 来自雨生红球藻，以及其他六个类胡萝卜素合成途径基因被共整合到酵母宿主的基因组中。这三个藻类基因中的每一个都比来自红发夫酵母的真菌基因显示出更高的将β-胡萝卜素转化为下游类胡萝卜素的效率。此外，含有 Hpchyb 的菌株比整合了 Crchyb 或 Czchyb 的菌株表现出更高的类胡萝卜素生产力，表明 Hpchyb 比 Crchyb 和 Czchyb 更有效。这些结果表明，β-胡萝卜素羟化酶在类胡萝卜素的生物合成中起着关键作用。
