de Almeida Fernanda Martins, Marques Suelen Adriani, Ramalho Bruna dos Santos, Massoto Tamires Braga, Martinez Ana Maria Blanco
Laboratório de Neurodegeneração e Reparo, Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil Universidade Federal do Rio de Janeiro, campus Macaé, Rio de Janeiro, Brasil.
Instituto de Biologia - Departamento de Neurobiologia - Laboratório de Regeneração Neural e Função - UFF - Rio de Janeiro, Brazil.
Restor Neurol Neurosci. 2015;33(1):43-55. doi: 10.3233/RNN-140431.
Despite substantial advances in surgical care and rehabilitation, the consequences of spinal cord injury (SCI) continue to present major challenges. Here we investigate whether transplantation of mesenchymal stem cells (MSCs) in mice during the chronic stage of SCI has benefits in terms of morphological and functional outcomes.
Mice were subjected to laminectomy at the T9 level, followed by a 1 minute spinal cord compression with a vascular clip. Four weeks later, 8 × 105 MSCs obtained from GFP mice were injected into the injury site. After eight weeks the analyses were performed.
The spinal cords of MSC-treated animals exhibited better white-matter preservation, greater numbers of fibers, higher levels of trophic factor expression, and better ultrastructural tissue organization. Furthermore, transplanted MSCs were not immunoreactive for neural markers, indicating that these cells mediate functional recovery through a paracrine effect, rather than by transforming into and replacing damaged glia in the spinal cord. MSC-treated mice also showed better functional improvement than control animals.
We conclude that MSC-based cell therapy, even when applied during the chronic phase of SCI, leads to changes in a number of structural and functional parameters, all of which indicate improved recovery.
尽管手术治疗和康复取得了重大进展，但脊髓损伤（SCI）的后果仍然带来重大挑战。在此，我们研究在SCI慢性期给小鼠移植间充质干细胞（MSC）在形态和功能结果方面是否有益。
小鼠在T9水平进行椎板切除术，随后用血管夹对脊髓进行1分钟压迫。四周后，将从绿色荧光蛋白（GFP）小鼠获得的8×105个MSC注射到损伤部位。八周后进行分析。
接受MSC治疗的动物脊髓显示出更好的白质保存、更多的纤维数量、更高水平的营养因子表达以及更好的超微结构组织。此外，移植的MSC对神经标志物无免疫反应性，表明这些细胞通过旁分泌作用介导功能恢复，而不是通过转化并替代脊髓中受损的神经胶质细胞。接受MSC治疗的小鼠在功能改善方面也比对照动物更好。
我们得出结论，基于MSC的细胞疗法，即使在SCI慢性期应用，也会导致一些结构和功能参数的变化，所有这些都表明恢复有所改善。
