Akande Motunrayo G, Aliu Yusuf O, Ambali Suleiman F, Ayo Joseph O
Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna, Nigeria.
Toxicol Ind Health. 2016 Jul;32(7):1328-34. doi: 10.1177/0748233714560394. Epub 2014 Dec 23.
The aim of this study was to investigate the effects of taurine (TA) on serum lipid profiles following chronic coadministration of chlorpyrifos (CP) and lead acetate (Pb) in male Wistar rats. Fifty rats randomly distributed into five groups served as subjects. Distilled water (DW) was given to DW group, while soya oil (SO; 1 mL kg(-1)) was given to SO group. The TA group was treated with TA (50 mg kg(-1)). The CP + Pb group was administered sequentially with CP (4.25 mg kg(-1); 1/20th median lethal dose (LD50)) and Pb at 233.25 mg kg(-1) (1/20th LD50), while the TA + CP + Pb group received TA (50 mg kg(-1)), CP (4.25 mg kg(-1)), and Pb (233.25 mg kg(-1)) sequentially. The treatments were administered once daily by oral gavage for 16 weeks. The rats were euthanised, and the blood samples were collected at the termination of the study. Sera obtained from the blood samples were analyzed for total cholesterol, high-density lipoprotein cholesterol, triglycerides, and malondialdehyde, and also the activities of serum antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase were analyzed. The low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, and atherogenic index were calculated. The results showed that CP and Pb induced alterations in the serum lipid profiles and evoked oxidative stress. TA alleviated the disruptions in the serum lipid profiles of the rats partially by mitigating oxidative stress. It was concluded that TA may be used for prophylaxis against serum lipid disorders in animals that were constantly co-exposed to CP and Pb in the environment.
本研究旨在探讨牛磺酸（TA）对雄性Wistar大鼠长期联合给予毒死蜱（CP）和醋酸铅（Pb）后血清脂质谱的影响。将50只大鼠随机分为五组作为研究对象。蒸馏水（DW）组给予蒸馏水，而大豆油（SO；1 mL kg⁻¹）组给予大豆油。TA组用TA（50 mg kg⁻¹）进行处理。CP + Pb组依次给予CP（4.25 mg kg⁻¹；半数致死剂量（LD50）的1/20）和Pb 233.25 mg kg⁻¹（LD50的1/20），而TA + CP + Pb组依次接受TA（50 mg kg⁻¹）、CP（4.25 mg kg⁻¹）和Pb（233.25 mg kg⁻¹）。通过口服灌胃每天给药一次，持续16周。在研究结束时对大鼠实施安乐死并采集血样。对从血样中获得的血清进行总胆固醇、高密度脂蛋白胆固醇、甘油三酯和丙二醛分析，同时分析血清抗氧化酶包括超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。计算低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和动脉粥样硬化指数。结果表明，CP和Pb诱导血清脂质谱改变并引发氧化应激。TA通过减轻氧化应激部分缓解了大鼠血清脂质谱的紊乱。得出的结论是，TA可用于预防在环境中持续共同暴露于CP和Pb的动物的血清脂质紊乱。
