Liu Handeng, Ding Songtao, Qin Qizhong, Tang Jun, Liu Li, Peng Huimin
Experimental Teaching Center, Chongqing Medical University, Yuzhong, Chongqing, 400016, People's Republic of China,
Parasitol Res. 2015 Mar;114(3):983-8. doi: 10.1007/s00436-014-4264-3. Epub 2014 Dec 25.
A new microsporidium was isolated from Histia rhodope Cramer (Lepidoptera, Zygaenidae), a pest of Bischofia javanica BL. in China. The morphology and molecular systematic of this novel microsporidian isolate had been described in this study. The spores were long oval and measured 3.1 × 1.9 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: 14-15 polar filament coils, posterior vacuole, and a diplokaryon. The sequenced rRNA gene of this isolate is 4309 bp long. The organization of the rRNA gene is 5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3', which is similar to that of other Nosema species (such as Nosema bombycis). Phylogenetic analysis based on LSU rRNA gene and SSU rRNA gene both revealed that this novel micorsporidian which isolated from H. rhodope had close relationship to the genus Nosema. Additionally, this isolate can also cause systemic infection of Bombyx mori. So, we should pay attention not only to N. bombycis, but also to other microsporidian (such as Nosema sp. HR) in sericulture in the future.
从中国重阳木锦斑蛾（鳞翅目，斑蛾科）——重阳木的一种害虫体内分离出一种新的微孢子虫。本研究描述了这种新型微孢子虫分离株的形态学和分子系统学特征。新鲜涂片上的孢子呈长椭圆形，大小为3.1×1.9μm。孢子的超微结构具有微孢子虫属的特征：14 - 15圈极丝、后液泡和双核。该分离株的rRNA基因测序长度为4309 bp。rRNA基因的组织形式为5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3'，与其他微孢子虫属物种（如家蚕微孢子虫）相似。基于LSU rRNA基因和SSU rRNA基因的系统发育分析均表明，从重阳木锦斑蛾分离出的这种新型微孢子虫与微孢子虫属关系密切。此外，该分离株还可引起家蚕的全身性感染。因此，未来在养蚕业中，我们不仅要关注家蚕微孢子虫，还要关注其他微孢子虫（如重阳木微孢子虫HR）。
