Xing Dongxu, Yang Qiong, Liao Sentai, Han Lanzhi, Li Qingrong, Zhao Chaoyi, Xiao Yang, Ye Mingqiang
Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, No. 133 Dongguanzhuang Yiheng Road, Guangzhou, 510610, China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Parasitol Res. 2017 Oct;116(10):2741-2746. doi: 10.1007/s00436-017-5584-x. Epub 2017 Aug 16.
A new microsporidium was isolated from Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), one of the most important rice pests in China. The morphology and molecular systematics of this novel microsporidium were described in this study. The spores were long oval and measured 3.17 × 1.64 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: a diplokaryon, 10-12 polar filament coils of the same type, and posterior vacuole. Small subunit rRNA gene sequence data and phylogenetic analysis further confirmed that the microsporidian species from C. suppressalis belong to the true Nosema sub-group of the genus Nosema. Besides, the microsporidium Nosema sp. CS could cause systemic infection of Bombyx mori and infect silkworms through vertical transmission. Therefore, mulberry field pest control should be carefully monitored, and sanitation of mulberry leaves is essential to control the pebrine disease in sericulture.
从中国最重要的水稻害虫之一二化螟（Chilo suppressalis (Walker)，鳞翅目：螟蛾科）中分离出一种新的微孢子虫。本研究描述了这种新型微孢子虫的形态和分子系统学。新鲜涂片上的孢子呈长椭圆形，大小为3.17×1.64μm。孢子的超微结构具有微孢子虫属的特征：双核，10 - 12个相同类型的极丝盘绕，以及后液泡。小亚基rRNA基因序列数据和系统发育分析进一步证实，来自二化螟的微孢子虫物种属于微孢子虫属的真微孢子虫亚组。此外，微孢子虫Nosema sp. CS可引起家蚕的全身感染，并通过垂直传播感染蚕。因此，应仔细监测桑园害虫防治情况，桑叶的卫生状况对于控制养蚕业中的微粒子病至关重要。
