Jiangsu University of Science and Technology, Zhenjiang 212018, China.
J Invertebr Pathol. 2011 Feb;106(2):339-42. doi: 10.1016/j.jip.2010.10.005. Epub 2010 Oct 28.
We studied the spore morphology and molecular systematics of a novel microsporidian isolate from Phyllobrotica armata Baly collected in China. The spores were long-oval and measured 4.7 × 2.6 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: 13-14 polar filament coils, posterior vacuole, and a diplokaryon. The complete rRNA gene sequence of the isolate was 4308 bp long. The organization of the rRNA gene was 5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3', which corresponds to that of the Nosema species. Phylogenetic analysis based on the rRNA gene sequence indicated that this isolate, designated as Nosema sp. PA, is closely related to Nosema bombycis and is correctly assigned to the "true"Nosema group.
我们研究了从中国采集的 Phyllobrotica armata Baly 中分离出的一种新型微孢子虫的孢子形态和分子系统发育。新鲜涂片上的孢子呈长椭圆形，大小为 4.7×2.6μm。孢子的超微结构特征为 Nosema 属：13-14 圈极丝、后液泡和双核。该分离株的完整 rRNA 基因序列长 4308bp。rRNA 基因的组织为 5'- LSU rRNA-ITS-SSU rRNA-IGS-5S-3'，与 Nosema 物种相对应。基于 rRNA 基因序列的系统发育分析表明，该分离株被命名为 Nosema sp. PA，与 Nosema bombycis 密切相关，并被正确归入“真正的”Nosema 组。
