Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China ; Department of Anesthesiology, Stomatological Hospital, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Neural Regen Res. 2012 Oct 25;7(30):2355-61. doi: 10.3969/j.issn.1673-5374.2012.30.005.
Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.
最近的研究表明，茶多酚能够穿过血脑屏障，抑制细胞凋亡，对脑缺血起到神经保护作用。此外，茶多酚还可以减少自由基对 DNA 的损伤。我们假设茶多酚可以在全脑缺血再灌注期间修复 DNA 损伤并抑制神经元凋亡。为了验证这一假设，我们采用了全脑缺血再灌注大鼠模型。结果表明，再灌注即刻腹腔内注射茶多酚可显著减少海马 CA1 区的细胞凋亡；这种作用在再灌注后 6 小时开始显现。免疫组化染色显示，茶多酚可逆转缺血再灌注诱导的 DNA 修复蛋白 X 射线修复交叉互补蛋白 1 和脱嘌呤/脱嘧啶内切酶/氧化还原因子-1 的表达减少，这一作用在 2 小时时开始出现。这两种作用至少持续 72 小时。这些实验结果表明，茶多酚可以促进 DNA 损伤修复，防止大脑细胞凋亡。
