Li Rongpeng, Zi Xiaoli, Wang Xinfeng, Zhang Xia, Gao Haofeng, Hu Nan
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian, 223300 People's Republic of China.
Springerplus. 2013 Jul 27;2:346. doi: 10.1186/2193-1801-2-346. eCollection 2013.
The isolation and characterization of a novel halophilic denitrifying marine bacterium is described. The halophilic bacterium, designated as NY-4, was isolated from soil in Yancheng City, China, and identified as Marinobacter hydrocarbonoclasticus by 16S rRNA gene sequence phylogenetic analysis. This organism can grow in NaCl concentrations ranging from 20 to 120 g/L. Optimum growth occurs at 80 g/L NaCl and pH 8.0. The organism can grow on a broad range of carbon sources and demonstrated efficient denitrifying ability (94.2% of nitrate removal and 80.9% of total nitrogen removal in 48 h). During denitrification by NY-4, no NO2 (-)-N was accumulated, N2 was the only gaseous product and no harmful N2O was produced. Because of its rapid denitrification ability, broad carbon use range and ability to grow under high salinity and pH conditions, NY-4 holds promise for the treatment of saline waste waters.
本文描述了一种新型嗜盐反硝化海洋细菌的分离与鉴定。该嗜盐细菌被命名为NY-4，从中国盐城市的土壤中分离得到，并通过16S rRNA基因序列系统发育分析鉴定为破环海洋杆菌。该菌株能在20至120 g/L的NaCl浓度范围内生长。最适生长条件为NaCl浓度80 g/L，pH 8.0。该菌株能利用多种碳源生长，并表现出高效的反硝化能力（48小时内硝酸盐去除率达94.2%，总氮去除率达80.9%）。在NY-4进行反硝化过程中，没有积累NO2 (-)-N，唯一的气态产物是N2，且不产生有害的N2O。由于其快速的反硝化能力、广泛的碳源利用范围以及在高盐和高pH条件下生长的能力，NY-4在处理含盐废水方面具有应用前景。
