Li Jiang-Yan, Wang Xiao-Bing, Luo Jian-Guang, Kong Ling-Yi
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China
J Chromatogr Sci. 2015 Aug;53(7):1131-9. doi: 10.1093/chromsci/bmu175. Epub 2014 Dec 23.
The fingerprints of Rhizoma coptidis (Huanglian) collected in different growing periods were established by high-performance liquid chromatography (HPLC), and the seasonal variations of six alkaloids, namely berberine, palmatine, jateorrhizine, coptisine, epiberberine and columbamine were assessed. In order to highlight the differences of their HPLC fingerprints, three chemometrics methods including Spearman's rank correlation, hierarchical clustering analysis (HCA) and principal component analysis were applied. The anti-inflammatory activity of R. coptidis collected in different developing stages was measured by NO inhibition assay. The relationship between HPLC fingerprints and anti-inflammatory activity named spectrum-effect relationship was investigated by partial least squares regression. The anti-inflammatory activity was positively correlated with the alkaloid contents. Berberine, palmatine, coptisine, jateorrhizine and epiberberine were the main anti-inflammatory components on the basis of the quantitative determination of the six alkaloids and spectrum-effect investigation. The collecting time of R. coptidis impacted the plant's alkaloid contents and its anti-inflammatory activity.
采用高效液相色谱法（HPLC）建立不同生长时期黄连的指纹图谱，并评估小檗碱、巴马汀、药根碱、黄连碱、表小檗碱和非洲防己碱6种生物碱的季节性变化。为突出其HPLC指纹图谱的差异，应用了Spearman秩相关、层次聚类分析（HCA）和主成分分析3种化学计量学方法。通过NO抑制试验测定不同发育阶段采集的黄连的抗炎活性。采用偏最小二乘回归研究HPLC指纹图谱与抗炎活性之间的关系，即谱效关系。抗炎活性与生物碱含量呈正相关。基于6种生物碱的定量测定和谱效研究，小檗碱、巴马汀、黄连碱、药根碱和表小檗碱是主要的抗炎成分。黄连的采收时间影响其生物碱含量和抗炎活性。
