Umei Namiko, Ono Takeya, Oki Sadaaki, Otsuka Akira, Otao Hiroshi, Tsumiyama Wakako, Tasaka Atsushi, Ishikura Hideki, Aihara Kazuki, Sato Yuta, Shimizu Michele Eisemann
Department of Physical Therapy, Faculty of Health and Welfare, Prefectural University of Hiroshima, Japan.
Program in Biological System Sciences, Graduate School of Comprehensive Scientific Research, Prefectural University of Hiroshima, Japan.
J Phys Ther Sci. 2014 Dec;26(12):1891-3. doi: 10.1589/jpts.26.1891. Epub 2014 Dec 25.
[Purpose] This study aimed to determine whether muscle atrophy induced by ischemic reperfusion injury in rats can be prevented by the administration of antioxidants and exercise. [Subjects] Rats were randomly divided into five groups: non-treated, ischemic, exercise, ascorbic acid and exercise, and tocopherol and exercise. [Methods] The relative weight ratio of the soleus muscle and the length of the soleus muscle fiber cross-section minor axis were used for the evaluation of muscle atrophy. Pain was assessed as the weight-bearing ratio of the ischemic side. A multiple comparison test and the paired t-test were used for the statistical analyses. [Results] Compared with the non-treated group, the relative weight ratios of the soleus muscle and the lengths of the soleus muscle fiber cross-section minor axis significantly decreased in the other groups. Excluding the non-treated group, the relative weight ratios of the soleus muscle were heaviest in the tocopherol and exercise group. Excluding the non-treated group, the lengths of the soleus muscle fiber cross-section minor axis were longest in the tocopherol and exercise group, followed by the ischemic, exercise, and ascorbic acid and exercise groups. The amount of antioxidant substances did not decrease on the weight-bearing ratio of the ischemic side. [Conclusion] In this study, using an experimental rat model, we confirmed that antioxidants and exercise effect muscle atrophy induced by ischemic reperfusion. The results show that muscle regeneration was facilitated by phagocytosis in the tocopherol and exercise group.
[目的]本研究旨在确定给予抗氧化剂和进行运动是否可以预防大鼠缺血再灌注损伤诱导的肌肉萎缩。[对象]大鼠被随机分为五组：未处理组、缺血组、运动组、抗坏血酸与运动组以及生育酚与运动组。[方法]比目鱼肌的相对重量比和比目鱼肌纤维横截面短轴长度用于评估肌肉萎缩。疼痛通过缺血侧的负重比进行评估。采用多重比较检验和配对t检验进行统计分析。[结果]与未处理组相比，其他组比目鱼肌的相对重量比和比目鱼肌纤维横截面短轴长度均显著降低。排除未处理组，生育酚与运动组比目鱼肌的相对重量比最重。排除未处理组，生育酚与运动组比目鱼肌纤维横截面短轴长度最长，其次是缺血组、运动组和抗坏血酸与运动组。抗氧化物质的量对缺血侧的负重比没有降低作用。[结论]在本研究中，使用实验大鼠模型，我们证实抗氧化剂和运动对缺血再灌注诱导的肌肉萎缩有影响。结果表明，生育酚与运动组中吞噬作用促进了肌肉再生。
