Bai Zhen-Min, Wang Zhe-Pei, Guo Ming-Hang, Zhang Ling-Jie, Xu Ji-Lai, Yu Liang, Li Chun-Ri
College of Sport and Rehabilitation, Beijing Sport University, Beijing 100084, China.
School & Hospital of Stomatology, Wuhan University, Wuhan 420079.
Zhen Ci Yan Jiu. 2020 Sep 25;45(9):720-5. doi: 10.13702/j.1000-0607.190545.
To observe the efficacy of electroacupuncture (EA) plus passive stretch exercise in the treatment of disused atrophy of gastrocnemius and soleus muscles in mice.
Fifty C57BL/6 mice were randomly and equally divided into 5 groups: blank control, model, passive stretch exercise (exercise), EA and EA+exercise groups. The muscular atrophy model was established by fixing the gastrocnemius and soleus muscles with plaster immobilization (by putting the right leg into a plastic vial and then twining the vial with medical plaster bandage from the ankle upwards to the thigh and groin to maintain the knee-joint flexion and ankle joint plantar flexion for 7 days). EA (2 Hz/100 Hz, 1 mA)was applied to bilateral "Zusanli"(ST36) for 10 min, once a day for 4 weeks. For mice with the passive exercise, the plastic vial was removed first, followed by pulling out the hindleg to seize the toes to stretch them until the right hindleg is fully extended, then, pushed the leg towards the body. The procedures were repeated once again and again for 10 min. The exercise was conducted once daily, for 4 weeks. The cross-sectional area of fast and slow muscle fibers of the soleus and gastrocnemius was measured under electronic microscope after ATPase histochemical stain and the expression of slow skeletal muscle troponin (TNNI1) and fast skeletal muscle troponin (TNNI2) in the soleus and gastrocnemius was detected by Western blot.
Compared with the blank control group, the cross-sectional areas of the fast and slow muscle fibers of the soleus and gastrocnemius muscles were significantly decreased in the model group (<0.05, <0.01). Following the interventions, the cross-sectional areas of the fast and slow muscle fibers of soleus muscle in the EA+exercise group, and those of the fast and slow muscle fibers of the gastrocnemius muscle in the EA and EA+exercise groups, and the expression levels of TNNI1 and TNNI2 proteins in the gastrocnemius muscle of the EA+exercise group were significantly increased in comparison with the model group (<0.05, <0.01).
EA combined with passive stretch exercise can promote the recovery of the soleus and gastrocnemius muscles in disused muscle atrophy mice, which may be related to its effect in up-regulating the expression of TNNI1 and TNNI2 proteins.
观察电针联合被动拉伸运动对小鼠比目鱼肌和腓肠肌废用性萎缩的治疗效果。
将50只C57BL/6小鼠随机等分为5组：空白对照组、模型组、被动拉伸运动组（运动组）、电针组和电针+运动组。通过石膏固定（将右下肢放入塑料瓶中，然后用医用石膏绷带从踝关节向上缠绕至大腿和腹股沟，保持膝关节屈曲和踝关节跖屈7天）建立肌肉萎缩模型。电针双侧“足三里”（ST36），频率为2Hz/100Hz，电流1mA，持续10分钟，每天1次，共4周。对于进行被动运动的小鼠，先移除塑料瓶，然后拉出后肢抓住脚趾进行拉伸，直至右后肢完全伸直，然后将腿向身体方向推。该操作重复进行10分钟。运动每天进行1次，共4周。经ATP酶组织化学染色后，在电子显微镜下测量比目鱼肌和腓肠肌快、慢肌纤维的横截面积，并通过蛋白质免疫印迹法检测比目鱼肌和腓肠肌中慢肌肌钙蛋白（TNNI1）和快肌肌钙蛋白（TNNI2）的表达。
与空白对照组相比，模型组比目鱼肌和腓肠肌快、慢肌纤维的横截面积均显著减小（<0.05，<0.01）。干预后，与模型组相比，电针+运动组比目鱼肌快、慢肌纤维的横截面积，电针组和电针+运动组腓肠肌快、慢肌纤维的横截面积，以及电针+运动组腓肠肌中TNNI1和TNNI2蛋白的表达水平均显著增加（<0.05，<0.01）。
电针联合被动拉伸运动可促进废用性肌肉萎缩小鼠比目鱼肌和腓肠肌的恢复，这可能与其上调TNNI1和TNNI2蛋白表达的作用有关。
